Journal of Medicinal Food, 2010, 13(4):815-20.

The antioxidant effects of genistein are associated with AMP-activated protein kinase activation and PTEN induction in prostate cancer cells

Chang Eun Park, Sung-Soo Kim, et al

Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea; et al

Epidemiological evidence suggests a lower incidence of prostate cancer in Asian countries, where soy products are more frequently consumed than in Western countries, indicating that isoflavones from soy have chemopreventive activities in prostate cells. Here, we tested the effects of the soy isoflavone genistein on antioxidant enzymes in DU145 prostate cancer cells. Genistein significantly decreased reactive oxygen species levels and induced the expression of the antioxidant enzymes manganese (Mn) superoxide dismutase (SOD) and catalase, which were associated with AMP-activated protein kinase (AMPK) and phosphatase and tensin homolog deleted from chromosome 10 (PTEN) pathways. The induced expression of catalase, MnSOD, and PTEN were attenuated by pretreatment with a pharmacological inhibitor for AMPK, indicating the effects of genistein primarily depend on AMPK. Furthermore, PTEN is essential for genistein activity, as shown by PTEN transfection in PTEN-deficient PC3 cells. Thus, genistein induces antioxidant enzymes through AMPK activation and increased PTEN expression.

美国《药膳杂志》，2010年8月

在前列腺癌细胞中染料木黄酮的抗氧化作用与AMP活化蛋白激酶的激活和诱导抑癌基因 PTEN

朴灿恩，金圣柱等

韩国庆熙大学研究生院中西医结合系等

流行病学证据表明在亚洲国家患前列腺癌的发病率较低，这些国家中豆制品的消耗比在西方国家更频繁，说明从大豆中提取的异黄酮对前列腺细胞具有化学防癌活性。这里，我们在前列腺癌细胞DU145中测试大豆异黄酮金雀异黄素对抗氧化酶的影响。染料木黄酮显著降低了活性氧的水平，并且诱导抗氧化剂酶锰 (Mn) 超氧化物歧化酶 (SOD) 和过氧化氢酶活性的表达，这些酶均与AMP活化的蛋白激酶 (AMPK) 和从染色体10(PTEN) 通路删除的磷酸酶和张力蛋白等同素异形体有关。用药理抑制剂预处理AMPK会减弱过氧化氢酶、 MnSOD和 PTEN 基因的诱导性表达，说明染料木黄酮的影响主要取决于 AMPK 。此外，PTEN 对于金雀异黄素的活性是必要的，就像缺失了PTEN抑癌基因的 PC3 细胞PTEN转染所示的那样。因此，金雀异黄素通过 AMPK 激活和增强PTEN 抑癌基因表达来诱导抗氧化酶。