Cancer Genomics & Proteomics, 2010, 7(3):111-28.

Use of cross species genomic profiling identifies pathways and genes differentially regulated in prostate cancer cells treated with soy protein isolates or purified genistein

MICHAEL A. LISS, MILTON W. DATTA, et al

Department of Urology, University of California Irvine, Irvine, CA, U.S.A.; et al

BACKGROUND: The main purified compound from soy protein isolates is genistein, but this purified phytoestrogen fails to recapitulate all the features of the soy-based diet that is associated with lower incidence of prostate cancer.

MATERIALS AND METHODS: Rat and human prostate cancer cell lines were treated with either soy protein isolates or purified genistein. In vitro cell growth was correlated with the associated genomic expression profiles using cDNA arrays. The data was subsequently bioinformatically analyzed within and across species to identify common changes in expression profiles associated with the soy protein or genistein treatments.

RESULTS: Gene expression profiling and data mining noted genes specific to soy; however, biological pathways showed the same gene regulation profiles between genistein and soy.

CONCLUSION: Genistein is likely the major contributor to the effect of soy proteins on cellular pathways; however, the expression of different genes using soy protein isolates suggests complexity in the many compounds found in whole soy protein.

希腊《肿瘤基因组学和蛋白质组学》，2010年6月

在用大豆蛋白分离物或纯化金雀异黄素治疗前列腺癌细胞时使用跨物种基因组分析来确认差异性调节的通路和基因

迈克尔 A 利斯，弥尔顿 W 达塔等

美国加利福尼亚大学欧文分校泌尿外科等

背景︰从大豆蛋白分离出来纯化的化合物主要是金雀异黄素，但是这种纯净的植物雌激素不能复制以大豆为基础与降低前列腺癌的发病率相关联的饮食习惯。

材料和方法︰ 用大豆蛋白分离物或纯化的染料木黄酮来处理大鼠和人类的前列腺癌细胞株。体外细胞生长与使用 cDNA 阵列的相关基因组表达谱呈正相关。随后用物种内和跨物种命名性分析这些数据来确认与大豆蛋白或染料木黄酮治疗相关表达谱的常见变化。

结果︰ 基因表达谱分析和数据挖掘指出大豆的特定基因，然而，生物学通路显示出染料木素和大豆之间相同的调节基因。
结论︰ 染料木黄酮可能是大豆蛋白对细胞通路发挥作用的主要贡献者。然而，使用大豆蛋白分离物表达的不同基因表现出在整个大豆蛋白中发现的许多化合物的复杂性。