Journal of Korean Medical Science, 2011, 26(11):1489-94.

Apoptotic Effects of Genistein, Biochanin-A and Apigenin on LNCaP and PC-3 Cells by p21 through Transcriptional Inhibition of Polo-like Kinase-1

Young Jin Seo, et al

Department of Urology, College of Medicine, Dongguk University, Gyeongju, Korea; et al

Natural isoflavones and flavones are important dietary factors for prostate cancer prevention. We investigated the molecular mechanism of these compounds (genistein, biochanin-A and apigenin) in PC-3 (hormone-independent/p53 mutant type) and LNCaP (hormone-dependent/p53 wild type) prostate cancer cells. A cell growth rate and apoptotic activities were analyzed in different concentrations and exposure time to evaluate the antitumor activities of genistein, biochanin-A and apigenin. The real time PCR and Western blot analysis were performed to investigate whether the molecular mechanism of these compounds are involving the p21 and PLK-1 pathway. Apoptosis of prostate cancer cells was associated with p21 up-regulation and PLK-1 suppression. Exposure of genistein, biochanin-A and apigenin on LNCaP and PC-3 prostate cancer cells resulted in same pattern of cell cycle arrest and apoptosis. The inhibition effect for cell proliferation was slightly greater in LNCaP than PC-3 cells. In conclusion, flavonoids treatment induces up-regulation of p21 expression, and p21 inhibits transcription of PLK-1, which promotes apoptosis of cancer cells.

韩国《韩国医学科学杂志》，2011年10月

金雀异黄素，鸡豆黄素A 和芹菜素通过 p21 对马球样激酶 1 的转录抑制使LNCaP 和PC-3细胞凋亡

徐真英等

韩国东国大学医学院泌尿科等

天然异黄酮和黄酮类化合物是重要的预防前列腺癌的膳食因素。我们研究了这些化合物 （金雀异黄素，鸡豆黄素 A 和芹菜素）在PC-3 （激素-独立/p53 突变型） 和 （激素-依赖/p53 野生型） LNCaP 前列腺癌细胞中的分子机制。在不同的浓度和暴露时间对金雀异黄素，鸡豆黄素 A 和芹菜素的抗肿瘤活性的细胞生长速率和凋亡活性进行了分析。实时聚合酶链反应和免疫印迹分析被用来探究这些化合物的分子机制是否包含 p21 和PLK-1 通路。前列腺癌细胞的凋亡与 p21 的上调和PLK-1的抑制有关。LNCaP 和 PC3前列腺癌细胞暴露于染料木素，鸡豆黄素 A 和芹菜素会导致相同模式的细胞周期中止和细胞凋亡。对细胞增殖的抑制作用 LNCaP 略大于 PC-3 细胞。总结，黄酮类化合物的治疗诱导了 p21 表达的上调和，而p21 抑制了PLK-1的转录，从而促进了肿瘤细胞凋亡。