Vivo, 2011, 25(6):917-21.

miRNAs Differentially Expressed in Prostate Cancer Cell Lines after Soy Treatment

Nadege Rabiau, et al

Department of Oncogenetics, Jean Perrin Centre, Clermont-Ferrand, France; et al

BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs that have aberrant expression in prostate cancer tissues. miRNAs are involved in the initiation and progression of cancer, and several miRNAs have been characterized as tumor suppressors or oncogenes. It has been shown that some miRNAs can be directly regulated from their own promoters by epigenetic alterations in cancer cells. Moreover, phytoestrogens are known to have epigenetic action on gene transcription. Hence, we conducted here an examination of the miRNA expression profile in human prostate cancer cell lines after soy phytoestrogen treatment.

MATERIALS AND METHODS: The comparative miRNA expression profiles of prostate cell lines (PC-3, DU145, LNCaP) after a 48-h treatment of 40 μM genistein, 110 μM daidzein, or 2 μM 5-azacytidine (5-AZA, a demethylating agent) were conducted with a Taqman low-density array.

RESULTS: We found that out of 377 miRNAs tested, 180, 170 and 150 miRNAs were amplified with 2% of variation in the triplicate in PC-3, DU145 and LNCap cells, respectively, and only 5 miRNAs for PC-3 and DU145 cells and 4 miRNAs for LNCap exhibited a significant change in their expression. Treatment with genistein or daidzein had similar effects on miRNA regulation to those of 5-AZA treatment.

CONCLUSION: This work demonstrated a new role of isoflavones on the regulation of miRNAs in prostate cancer.

希腊《体内》，2011年11月

经过大豆治疗后miRNAs在前列腺癌细胞中的差异性表达

纳德吉 罗宾奥等

法国克莱蒙费朗让佩林中心癌症部等

背景︰ 小分子 RNA (miRNA) 是一种分子量小的非编码 RNA， 在前列腺癌组织中被异常表达。miRNA 参与了癌症的激发和进展，并且几种miRNA 被确认作为肿瘤抑制因子或抑癌基因。已经证明，在癌细胞中通过表观遗传的改变一些miRNA可直接被自己的启动子调控。此外，植物雌激素对基因转录具有表观遗传活性是众所周知的。因此，我们在这里对经过大豆植物雌激素治疗后的人体前列腺癌细胞的 miRNA 的表达进行了实验。

材料和方法︰在用40 μM 染料木黄酮、110  μM大豆黄酮或 2 μM 5-氮杂胞苷 （5-AZA，一种甲基化试剂）对前列腺细胞 （PC-3、 DU145 ，LNCaP）进行 48 h 治疗后用Taqman 低密度数组对miRNA 的表达进行比较。

结果︰ 我们发现从377 mi RNA 测试中，在 PC- 3、 DU145 和 LNCap 细胞中180，170 和150 miRNA 被扩增了2%。只有PC -3 和 DU145 细胞 中的5mi RNA和LNCap中 4 mi RNA 在表达方面表现出显著变化。染料木素或大豆黄酮治疗对 miRNA 的调控具有与 5-AZA治疗相似的效果。

结论︰ 这项工作证明了异黄酮对前列腺癌的miRNA 调控的一个新作用。