Journal of Medicinal Food, 2011, 14(12):1597-603.

Genistein Selectively Inhibits Estrogen-Induced Cell Proliferation and Other Responses to Hormone Stimulation in the Prepubertal Rat Uterus

Leonardo Gaete, Andrei N. Tchernitchin, Rodrigo Bustamante, et al

Laboratory of Experimental Endocrinology and Environmental Pathology, Institute of Biomedical Sciences (ICBM), University of Chile Medical School, Santiago, Chile; et al

Sex hormone replacement therapy helps improve quality of life in climacteric women. However, estrogen-induced cell proliferation in the uterus and mammary gland increases the risk for cancer in these organs. The lower incidence of mammary cancer in Asian women than in western women has been attributed to high intake of soy isoflavones, including genistein. Our previous work in the prepubertal rat uterus model showed that genistein (0.5 mg/kg body weight subcutaneously) caused an estradiol-like hypertrophy in myometrial and uterine luminal epithelial cells and an increase in RNA content in luminal epithelium; however, it did not induce cell proliferation, uterine eosinophilia, or endometrial edema. The present study investigated, in the same animal model, the effect of genistein administration (0.5 mg/kg body weight subcutaneously) before treatment with estradiol-17β (0.33 mg/kg body weight subcutaneously) on uterine responses that were not induced by genistein. Pretreatment with this phytoestrogen completely inhibited estradiol-induced mitoses in uterine luminal epithelium, endometrial stroma, and myometrium and partially inhibited estradiol-induced uterine eosinophilia and endometrial edema. These findings indicate that genistein protects against estrogen-induced cell proliferation in the uterus and suggest that future studies should investigate the possibility of using this agent to decrease the risk for uterine cancer after hormone replacement therapy in climacteric women.

美国《药膳杂志》，2011年12月

在青春期前大鼠子宫中金雀异黄素有选择性地抑制雌激素诱导的细胞增殖和其他对激素刺激的反应

莱昂纳多 耶德，安德烈 N.邱妮奇，罗德里戈  巴斯塔曼特等

智利智利大学医学院研究所生物医学科学内分泌学和环境病理学实验室等

性激素替代疗法有助于提高更年期妇女的生活质量。然而，在子宫和乳腺组织中雌激素诱导的细胞增殖提高了这些器官癌变的风险。亚洲妇女比西方妇女乳腺癌的发病率低发展归功于大量摄入大豆异黄酮，包括染料木黄酮。我们以前在青春期前大鼠子宫模型中的工作表明，金雀异黄素 （0.5 毫克/千克体重皮下地） 在肌层和子宫腔上皮细胞中引起雌二醇样肥厚以及子宫腔上皮的RNA 含量增加，然而它不会引起细胞增殖、 子宫嗜酸粒细胞增多或子宫内膜水肿。本研究探讨了在相同的动物模型中，染料木黄酮给药（0.5 毫克/千克体重皮下）对用雌二醇 17-β （0.33 毫克/千克体重皮下注射）治疗子宫反应以前的影响。在子宫腔上皮细胞、 子宫内膜间质和肌层用植物雌激素预处理可以完全抑制雌二醇诱导的分裂，并且部分抑制雌二醇诱发的子宫嗜酸性粒细胞和子宫内膜水肿。这些结果表明，染料木黄酮可阻止子宫中雌激素诱导的细胞增殖，并且建议未来的研究应探讨使用此试剂降低接受过激素替代治疗更年期妇女患上子宫内膜癌风险的可能性。