通过金雀异黄素恢复宫颈癌细胞对顺铂的敏感性︰NFκB和 Akt/mTOR 信号转导途径的作用_沙欣

针对病种：宫颈癌

发表时间：2012年9月

发表国家：希腊

登载刊物：肿瘤学杂志

研究单位：土耳其埃拉泽幼发拉底河大学兽医学院动物营养系等

研究人员：K. 沙欣，M. 图兹朱，N. 巴斯克等

主要结论：本研究结果表明，金雀异黄素可以通过抑制 NF-κ B 和 Akt/mTOR 通路增强顺铂的活性。金雀异黄素是一种有前途的无毒营养药剂，可以提高顺铂治疗宫颈癌患者的疗效。需要进行金雀异黄素与顺铂联合用药的临床实验来验证这一假设.

Journal of Oncology, 2012(5):461562-461562.

Sensitization of Cervical Cancer Cells to Cisplatin by Genistein: The Role of NFκB and Akt/mTOR Signaling Pathways

K. Sahin, M. Tuzcu, N. Basak, et al

Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, 23119 Elazig, Turkey; et al

Cervical cancer is among the top causes of death from cancer in women. Cisplatin-based chemotherapy has been shown to improve survival; however, cisplatin treatment is associated with toxicity to healthy cells. Genistein has been used as an adjunct to chemotherapy to enhance the activity of chemotherapeutic agents without causing increased toxicity. The present study was designed to investigate the effect of genistein (25 μM) on antitumor activity of cisplatin (250 nM) on HeLa cervical cancer cells. We have examined the alterations in expression of NF-κB, p-mTOR, p-p70S6K1, p-4E-BP1, and p-Akt protein levels in response to treatment. The combination of 25 μM genistein with 250 nM cisplatin resulted in significantly greater growth inhibition (P < 0.01). Genistein enhanced the antitumor activity of cisplatin and reduced the expression of NF-κB, p-mTOR, p-p70S6K1, p-4E-BP1, and p-Akt. The results in the present study suggest that genistein could enhance the activity of cisplatin via inhibition of NF-κB and Akt/mTOR pathways. Genistein is a promising nontoxic nutritional agent that may enhance treatment outcome in cervical cancer patients when given concomitantly with cisplatin. Clinical trials of genistein and cisplatin combination are warranted to test this hypothesis.

希腊《肿瘤学杂志》，2012年9月

通过金雀异黄素恢复宫颈癌细胞对顺铂的敏感性︰NFκB和 Akt/mTOR 信号转导途径的作用

K. 沙欣，M. 图兹朱，N. 巴斯克等

土耳其埃拉泽幼发拉底河大学兽医学院动物营养系等

宫颈癌是患癌妇女死亡的主要原因之一。以顺铂为基础的化疗已被证实可以提高生存率；然而，顺铂治疗会毒害健康的细胞。金雀异黄素曾作为化疗的辅助手段，在不会导致毒性增加的情况下，增强化疗药物的活性。本研究旨在探讨金雀异黄素 (25 μ M) 对顺铂(250 nM)抗子宫颈癌 HeLa 细胞活性的影响。我们测试了经过治疗后NF-κ B、p-mTOR、 p-p70S6K1、 p-4E-BP1 和p-Akt 蛋白水平表达的改变。25 μM 染料木黄酮与 250nM顺铂联合导致明显更强的生长抑制作用 (P < 0.01)。金雀异黄素增强顺铂的抗肿瘤活性和并降低NF-κB、p-mTOR、 p-p70S6K1、 p-4E-BP1和p-Akt 的表达。本研究结果表明，金雀异黄素可以通过抑制 NF-κ B 和 Akt/mTOR 通路增强顺铂的活性。金雀异黄素是一种有前途的无毒营养药剂，可以提高顺铂治疗宫颈癌患者的疗效。需要进行金雀异黄素与顺铂联合用药的临床实验来验证这一假设。