Molecular Medicine Reports, 2012, 5(2):567-9.

Clobetasol synergistically diminishes Ciz1 expression with genistein in U937 cells

Naoko Okumura, Hitomi Yoshida, Yuri Nishimura, et al

Department of Environmental Health Science, Nara Women's University, Nara 630-8506, Japan

Cip-interacting zinc finger protein 1 (Ciz1) stimulates DNA replication and has been implicated in the tumorigenesis of breast cancer cells. In order to investigate the possibility of using medicinal glucocorticoids against breast cancer, we studied whether certain glucocorticoids affect the expression of Ciz1. The in vitro effect of clobetasol treatment on the reduction of Ciz1 expression was detected by reverse transcriptase-polymerase chain reaction. Western blotting also confirmed the down-regulation of the protein in a dose-dependent manner upon clobetasol treatment in U937 monocytoid cells. Furthermore, we found that Ciz1 protein expression was decreased after pre-treatment of the cells with clobetasol and genistein. An extract of Lens culinaris also had a synergistic effect on the repression of Ciz1 protein expression.

希腊《分子医学报告》，2012年2月

在 U937 细胞中金雀异黄素协同氯倍他索减少Ciz1 的表达

奥村直子，吉田瞳，西村尤里，等

日本奈良女子大学环境健康科学部

Cip 相互作用的锌指状蛋白 1 (Ciz1) 刺激 DNA 复制并且与乳腺癌细胞癌变有牵连。为了探讨使用药用糖皮质激素治疗乳腺癌的可能性，我们研究了某些糖皮质激素是否影响 Ciz1 的表达。通过逆转录-聚合酶链反应测试氯倍他索体外治疗对减少 Ciz1 表达的影响。免疫印迹法也证实了氯倍他索治疗U937 单核细胞以剂量依赖的方式下调蛋白水平。此外，我们发现在用氯倍他索和金雀异黄素预处理细胞后, Ciz1 蛋白的表达下降。小扁豆的提取物对 Ciz1 蛋白表达的抑制也具有协同效应。