Cytotechnology, 2013, 65(6):973-8.

Genistein-induced G2/M cell cycle arrest of human intestinal colon cancer Caco-2 cells is associated with Cyclin B1 and Chk2 down-regulation

Junkyu Han, Yui Kurita, Hiroko Isoda

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan; Alliance for Research on North Africa (ARENA)University of Tsukuba, Tsukuba, Japan

Genistein is an isoflavonic phyto-oestrogen contained in soya beans. It is thought to display anti-cancer effects. This study was designed to investigate its effect on human intestinal colon cancer Caco-2 cells. MTT assay, flow cytometric analysis and western blotting were used to investigate the effect of genistein on cell proliferation, cell cycle progression and protein alterations of selected cell cycle-related proteins in Caco-2 cells. Our results showed that genistein and daidzein significantly suppressed cell proliferation. Genistein treatment was demonstrated to modulate cell cycle distribution through accumulation of cells at G2/M phase, with a significant decreasing effect of Cyclin B1 and Serine/threonine-protein kinase 2 (Chk2) proteins expression. However, daidzein did not alter the cell cycle progression in Caco-2 cells. All these observation strongly indicate that genistein has anti-proliferative effect in human intestinal colon cancer Caco-2 cells through the down-regulation of cell cycle check point proteins, Cyclin B1 and Chk2.

荷兰《养殖业》，2013年12月

金雀异黄素诱导人体结肠癌细胞Caco-2的 G2/M 期细胞周期中止与细胞周期蛋白 B1 和 Chk2 下调有关

韩俊尤，栗田唯，矶田裕子

日本筑波大学生命与环境科学研究院和北非研究同盟会

金雀异黄素是大豆中含有的一种异黄酮植物雌激素，被认为具有抗肿瘤作用。本研究旨在探讨其对人体肠道结肠癌 Caco-2 细胞的影响。用MTT测试、 流式细胞术分析法和免疫印迹法探究在 Caco-2 细胞中金雀异黄素对细胞增殖、 细胞周期进程和选定细胞周期相关蛋白蛋白质变化的作用。我们的研究结果表明，金雀异黄素和大豆黄酮能明显抑制细胞的增殖。通过细胞在 G2/M 期的积累和细胞周期蛋白 B1 和丝氨酸/苏氨酸蛋白激酶 2 (Chk2) 蛋白表达的明显下降，金雀异黄素的治疗调节了细胞周期分布。然而，大豆黄酮并没有改变 Caco-2 细胞的细胞周期进程。这些所有的现象都强烈表明，金雀异黄素对人类肠道结肠癌 Caco-2 细胞具有抗增殖作用，通过下调细胞周期检查点蛋白，细胞周期蛋白 B1 和 Chk2。