BMC Complementary and Alternative Medicine, 2013, 13(1):103-103.

Protective effect of genistein on radiationinduced intestinal injury in tumor bearing mice

Tae Gen Son, Eun Ji Gong, Min Ji Bae, et al

Research Center, Dongnam Institute of Radiological & Medical Sciences (DIRAMS), Jwadong-gil 40, Jangan-eup, Gijang-gun, Busan, Republic of Korea; Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Center, Chonnam National University, Gwangju, South Korea

Background

Radiation therapy is the most widely used treatment for cancer, but it causes the side effect of mucositis due to intestinal damage. We examined the protective effect of genistein in tumor-bearing mice after abdominal irradiation by evaluation of apoptosis and intestinal morphological changes.

Methods

Mouse colon cancer CT26 cells were subcutaneously injected at the flank of BALB/c mice to generate tumors. The tumor-bearing mice were treated with abdominal radiation at 5 and 10 Gy, and with genistein at 200 mg/kg body weight per day for 1 d before radiation. The changes in intestinal histology were evaluated 12 h and 3.5 d after irradiation. To assess the effect of the combination treatment on the cancer growth, the tumor volume was determined at sacrifice before tumor overgrowth occurred.

Results

Genistein significantly decreased the number of apoptotic nuclei compared with that in the irradiation group 12 h after 5 Gy irradiation. Evaluation of histological changes showed that genistein ameliorated intestinal morphological changes such as decreased crypt survival, villus shortening, and increased length of the basal lamina 3.5 d after 10 Gy irradiation. Moreover, the genistein-treated group exhibited more Ki-67-positive proliferating cells in the jejunum than the irradiated control group, and crypt depths were greater in the genistein-treated group than in the irradiated control group. The mean weight of the CT26 tumors was reduced in the group treated with genistein and radiation compared with the control group.

Conclusion

Genistein had a protective effect on intestinal damage induced by irradiation and delayed tumor growth. These results suggest that genistein is a useful candidate for preventing radiotherapy-induced intestinal damage in cancer patients.

英国《BMC 补充和替代医学》，2013年5月

金雀异黄素对荷瘤小鼠的辐射诱导肠道损伤的保护作用

孙泰英，孔恩智，裴珉姬，等

韩国釜山放射卫生与医学科学东南研究所 （迪拉姆）；韩国光州全南国立大学兽医学院和兽医医学研究中心兽医解剖学部

背景：

放射治疗是使用最广泛的治疗癌症的方法，但是由于肠道损伤会产生黏膜炎的副作用。通过测试细胞凋亡及肠道形态学变化，我们评价了金雀异黄素对经过放射治疗的荷瘤小鼠的保护作用。

方法：

小鼠结肠癌 CT26 细胞被皮下注射到BALB/c 小鼠的侧面以产生肿瘤。提前1天服用 200 毫克/公斤体重的金雀异黄素，再以 5 和 10 Gy强度进行腹部放射治疗荷瘤小鼠。肠道组织学的变化在照射后12 h 和 3.5 d天测试。为了评估联合疗法对肿瘤生长的作用，在肿瘤增生发生之前测定肿瘤体积。

结果：

与经过5Gy 照射12 h后的放射组相比，金雀异黄素明显降低了凋亡细胞核的数量。组织学变化测试表明，金雀异黄素改善了肠道的形态学变化，例如降低隐窝的生存率，缩短绒毛，并且 在10 Gy 照射3.5 d后增加了基膜的长度。在此基础上，金雀异黄素治疗组在空肠中表现出比放射对照组更多的 Ki-67- 阳性增殖细胞，并且隐窝的深度也更大。金雀异黄素治疗组CT26 肿瘤的平均重量也有所减少。

结论：