BioFactors, 2013, 39(3):279–293.

An Ex Vivo Study of Selenium, Genistein on the Morphological and Nuclear Changes in Anticancer Drug-Induced Apoptosis in Human Peripheral Blood Lymphocytes

Elango Sonaa, Subbiah Usha, Jeong Ja In

Department of Biomedical Sciences, College of Natural Sciences, Catholic University of Daegu, Hayang-eup, Gyeongsan-si, Gyeongbuk, Republic of Korea

Behavioral difference of cancer cells and normal cells to anticancer drugs is diverse. Debilitating side effects observed with certain chemotherapeutic and prophylactic anticancer drugs have lead to identification of natural agents that may offer protective role against the damage induced in normal cells. The present study aimed to determine the protective role of the micronutrient mineral selenium (Se) and plant product genistein (Gn) in synergy and single, against the apoptosis induced in human peripheral blood lymphocytes by the cytotoxic agents cisplatin and mitomycin C. We observed reduction in apoptotic potential of cisplatin and mitomycin C in lymphocytes by pre-treatment and post-treatment with selenium (30 μM) and genistein (100 μM). The intercellular interaction of Se + Gn in synergy is found to be more efficient in protecting lymphocytes than as individual. Moreover the beneficial results were well pronounced in pre-treatment than in post-treatment condition. The protective action of these natural compounds suggests that they might be useful immunomodulators during the course of chemotherapy and prophylaxis.

荷兰《生物因子》，2013年5月

在人体外周血淋巴细胞中当抗肿瘤药物诱导细胞凋亡时，硒和金雀异黄素对细胞核形态学上改变的体外研究

伊兰古 素娜，萨比阿 乌莎，尹真恩

大韩民国大邱天主教大学自然科学学院生物医学科学系

癌细胞和正常细胞对抗癌药物的行为差异是不同的。弱化某些化疗及预防性抗癌药物的副作用会导致天然药物的划分，可提供对正常细胞诱导损伤的保护作用。本研究旨在确定微量矿物质元素硒 (Se)和植物产物金雀异黄素(Gn) 协同和单独对人体外周血淋巴细胞由毒性药物顺铂和丝裂霉素 C诱导的细胞凋亡的保护作用。我们观察到通过用硒 (30 μM) 和金雀异黄素 (100 μM)前处理和后处理，顺铂和丝裂霉素 C 在淋巴细胞中细胞凋亡的潜力减少。Se + Gn 协同的细胞间相互作用比单独使用被发现能更有效的保护淋巴细胞。此外，明显前处理比后处理能够取得更有益的结果。这些天然化合物的保护性行为表明，在化疗及预防性治疗过程中它们可能是有用的免疫调节剂。