与个别大豆异黄酮相比，低剂量的金雀异黄素和大豆黄酮组合具有对同基因型的人体前列腺癌细胞的协同预防作用_董鑫

针对病种：前列腺癌

发表时间：2013年12月

发表国家：英国

登载刊物：食物化学

研究单位：美国特拉华大学动物和食品科学学院等

研究人员：董鑫，徐文清，罗伯特 A 赛克斯，等

主要结论：因此本研究的主要目的是确认金雀异黄素联合低水平的大豆苷元是一种更有效、更安全化学预防PCa的组合。大豆苷元和金雀异黄素表现出抑制PCa细胞增殖及诱导细胞凋亡的协同效应.

Food Chemistry, 2013, 141(3):1923-33.

Combination of low dose of genistein and daidzein has synergistic preventive effects on isogenic human prostate cancer cells when compared with individual soy isoflavone

Xin Dong, Wenqing Xu, Robert A. Sikes, et al

Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA; et al

The reduced incidence of prostate cancer (PCa) in Asia countries has been attributed to high soy diets, and major soy isoflavones, in particular daidzein and genistein, are thought to be the source of the beneficial and anti-cancer effects of soy foods. However, attention has been drawn to the safety of using high levels of soy isoflavones in humans, which is especially the concern for consumers taking regular soy isoflavone dietary supplements. The main objective of this study is thus to identify a soy isoflavone combination with lower levels of daidzein and genistein to be a more efficacious and safer chemo-preventive agent for PCa. The anticancer effects of daidzein and genistein, and their combinations on early-stage androgen-dependent PCa cells (LNCaP) and bone metastatic LNCaP-derivative PCa cells (C4-2B) were compared. Cells were treated with varying concentrations of daidzein, genistein (25-200 μM) or their combinations (25 or 50 μM) and cell proliferation, apoptosis, cell cycles and cellular uptakes of the isoflavones were measured after 48 h. Daidzein and genistein showed a synergistic effect on inhibiting cell proliferation and inducing apoptosis of both PCa cells. Twenty-five μM daidzein/50 μM genistein and 50 μM daidzein/50 μM genistein significantly increased the apoptotic effects on C4-2B cells although they did not show any effect when used individually. Except 50 μM daidzein/50 μM genistein, all other combinations had no impacts on cell cycles. For treatment with soy isoflavone combination, genistein was always better taken up than daidzein by both LNCaP and C4-2B cells.

英国《食物化学》，2013年12月

与个别大豆异黄酮相比，低剂量的金雀异黄素和大豆黄酮组合具有对同基因型的人体前列腺癌细胞的协同预防作用

董鑫，徐文清，罗伯特 A 赛克斯，等

美国特拉华大学动物和食品科学学院等

在亚洲国家前列腺癌 (PCa)风险的减少归功于高大豆饮食及其主要组分大豆异黄酮，特别是大豆苷元和金雀异黄素，被认为是大豆食品有益和抗肿瘤作用的来源。然而，注意力已被吸引到人体使用高浓度大豆异黄酮的安全性，特别是对定期摄取大豆异黄酮膳食补充剂的关注。因此本研究的主要目的是确认金雀异黄素联合低水平的大豆苷元是一种更有效、更安全化学预防PCa的组合。比较大豆苷元和金雀异黄素和它们的组合对雄激素依赖性 PCa细胞 (LNCaP) 早期阶段和骨转移 LNCaP 衍生 PCa 细胞 (C4-2B) 的抗癌影响。用不同浓度的大豆苷元、金雀异黄素 (25-200 μM) 或其组合物 (25 或 50 μM) 处理细胞，并且细胞增殖、凋亡、细胞周期和细胞对异黄酮的吸收在48 h后测定。大豆苷元和金雀异黄素表现出抑制PCa细胞增殖及诱导细胞凋亡的协同效应。25 μ M 大豆苷元/50 μ M 金雀异黄素和 50 μM 大豆苷元/50 μM 金雀异黄素显著增加了对 C4-2B 细胞凋亡的影响，虽然它们单独使用时并没有显示任何效果。除了 50 μM 大豆苷元/50 μM 的金雀异黄素，所有其他的组合对细胞周期没有影响。用大豆异黄酮组合治疗，在 LNCaP 和 C4-2B 细胞中金雀异黄素总是比大豆黄酮更好吸收。