The Journal of Membrane Biology, 2013, 246(4):307-314.

Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones

Vladimir Ajdžanović, et al

Department of CytologyInstitute for Biological Research “Siniša Stanković,” University of Belgrade, Belgrade, Serbia; et al

Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 μg/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 μg/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 ± 0.006 (LNCaP), 0.753 ± 0.010 (LNCaP + genistein), 0.723 ± 0.007 (PC-3) and 0.741 ± 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.

美国《膜生物学杂志》，2013年4月

在用大豆异黄酮治疗以后转移性前列腺癌的细胞膜流动性、 侵袭性和动力学表现

弗拉基米尔  阿甲德，等

塞尔维亚贝尔格莱德大学生物学研究学院细胞学系等

在前列腺癌的治疗中大豆异黄酮是一种有希望的非常规疗法。我们研究的目的是确定金雀异黄素和大豆黄酮在LNCaP和 PC-3 前列腺癌细胞中对反映转移潜能、 细胞膜流动性、 侵袭性和基底膜动态表型等参数的影响。进行细胞生命力测试，使用浓度范围广泛的大豆异黄酮 （72 小时6-75 μg/m)，测定其 IC50 浓度。进行前列腺癌细胞膜流动性的电子顺磁共振调查，在金雀异黄素和大豆黄酮的IC50 浓度分别为12.5 和 25 μg/m l， 10 分钟。金雀异黄素引起了膜顺序序参量 的显著提高（与细胞膜流动性相互成正比），0.722 ± 0.006 (LNCaP)，0.753 ± 0.010 （LNCaP + 金雀异黄素），0.723 ± 0.007 (PC-3) 和 0.741 ± 0.004 （PC-3 + 金雀异黄素）；然而，大豆黄酮没有观察到这样的效果。当金雀异黄素和大豆黄酮在各自的 IC50 浓度降低前列腺癌细胞的增殖时，在培养 的72 小时期间，只有金雀异黄素引发了对动态表型的影响并减弱了侵袭性。与 LNCaP 细胞相比PC-3 细胞的影响更加明显。我们的研究结果表明： （1） 侵袭活性至少部分地依赖于细胞膜的流动性，（2） 金雀异黄素通过改变前列腺癌细胞的力学性能可能产生抗转移的作用，和（3）大豆苷元应以比金雀异黄素更高的浓度使用以实现药理作用。