Molecular Medicine Reports, 2013, 7(3):781-784.

Antiproliferative activity of daidzein and genistein may be related to ERα/c-erbB-2 expression in human breast cancer cells

Eun Jeong Choi, Gun-Hee Kim

Plant Resources Research Institute, Duksung Women's University, Seoul 132-714, Republic of Korea

In this study, we investigated the antiproliferative activity of the isoflavones daidzein and genistein in three breast cancer cell lines with different patterns of estrogen receptor (ER) and c‑erbB‑2 protein expression (ERα‑positive MCF‑7 cells, c‑erbB‑2‑positive SK‑BR‑3 cells and ERα/c‑erbB‑2‑positive ZR‑75‑1). After treatment at various concentrations (1‑200 µM for 72 h), the effect of daidzein and genistein on the proliferation of different cell types varied; these effects were found to be associated with ERα and c‑erbB‑2 expression. Daidzein and genistein exhibited biphasic effects (stimulatory or inhibitory) on proliferation and ERα expression in MCF‑7 cells. Although 1 µM daidzein significantly stimulated cell growth, ERα expression was unaffected. However, genistein showed marked increases in proliferation and ERα expression after exposure to <10 µM genistein. Notably, the inhibition of cell proliferation by 200 µM genistein was greater compared to that by daidzein at the same concentration. Daidzein and genistein significantly inhibited proliferation of SK‑BR‑3 and ZR‑75‑1 cells in a dose‑dependent manner. In addition, ERα and c‑erbB‑2 expression was reduced by daidzein and genistein in both SK‑BR‑3 and ZR‑75‑1 cells in a dose‑dependent manner. However, the effect of genistein was greater compared to that of daidzein. In conclusion, the isoflavones daidzein and genistein showed anti‑breast cancer activity, which was associated with expression of the ERα and c‑erbB‑2 receptors.

希腊《分子医学报告》，2013年3月

在人体乳腺癌细胞中大豆苷元和金雀异黄素的抗增殖活性可能与 ERα/c-erbB-2 的表达有关

崔恩贞，金正熙

韩国首尔德成女子大学植物资源研究所

在此研究中，我们研究了异黄酮苷元和金雀异黄素在三种雌激素受体 (ER) 和 c‑erbB‑2 蛋白表达模式不同的乳腺癌细胞（ERα‑阳性 MCF‑7 细胞，c‑erbB‑2‑阳性 SK‑BR‑3 细胞和 ERα/c‑erbB‑2‑阳性 ZR‑75‑1）中的抗增殖活性。在不同浓度 (1‑200 µM，72 h) 治疗后，大豆苷元和金雀异黄素对不同类型细胞增殖的影响各不相同；发现这些影响与 ERα 和 c‑erbB‑2 的表达有关。大豆苷元和金雀异黄素对MCF‑7 细胞的增殖和ERα 表达表现出双向性作用 （刺激或抑制）。虽然 1 µM 大豆黄酮显著刺激细胞生长，ERα 的表达不受影响。然而，当接触< 10 µM 金雀异黄素以后，金雀异黄素在细胞增殖和ERα 表达表现出明显的提升。值得注意的是， 200 µM 金雀异黄素对细胞增殖的抑制作用比相同浓度下的大豆黄酮更大。大豆苷元和金雀异黄素以剂量依赖的方式明显抑制了SK‑BR‑3 和 ZR‑75‑1 细胞的增殖。此外，ERα 和 c‑erbB‑2 的表达被大豆苷元和金雀异黄素在 SK‑BR‑3 和 ZR‑75‑1 细胞中以剂量依赖的方式下调。然而，金雀异黄素的影响相比大豆黄酮较大。总之，异黄酮大豆苷元和金雀异黄素表现出抗乳腺癌活性，并且与 ERα 和 c‑erbB‑2 受体的表达有关。