Current Drug Targets, 2013, 14(10):1150-6.

Genistein Down-Regulates miR-223 Expression in Pancreatic Cancer Cells

Jia Ma, Long Cheng, Hao Liu, et al

Department of Biochemistry and Molecular Biology, Bengbu Medical College, Anhui, 233030, China

Although genistein has been shown to inhibit tumorigenesis in a variety of human cancers including pancreatic cancer (PC), the exact molecular mechanism of its anti-cancer effects has not yet been fully elucidated. Recently, microRNAs (miRNAs) have been reported to regulate multiple aspects of tumor development and progression, indicating that targeting miRNAs could be a novel strategy to treat human cancers. In the current study, we investigated whether a natural compound genistein could down-regulate onco-miR-223, resulting in the inhibition of cell growth and invasion, and induction of apoptosis in PC cells. We found that genistein treatment significantly inhibited miR-223 expression and up-regulated Fbw7, one of the targets of miR-223. Moreover, down-regulation of miR-223 inhibited cell growth and induced apoptosis in PC cells. These findings suggest that genistein exerts its anti-tumor activity partly through downregulation of miR-223 in PC cells.

荷兰《当代药物靶点》，2013年9月

在胰腺癌细胞中金雀异黄素下调 miR-223 的表达

马嘉，程龙，刘浩，等

中国安徽蚌埠医学院生化与分子生物学系

虽然金雀异黄素已被证明可以在各种人类癌症包括胰腺癌 (PC)抑制肿瘤发生，但是其抗肿瘤作用的精确分子机制尚未完全阐明。最近，小分子 RNA (miRNAs) 被报导可以调节肿瘤发展和进展的多个方面，预示着靶向miRNAs可能是一种治疗人体癌症的新策略。在当前的研究中，我们研究了天然混合物金雀异黄素能否下调onco-miR-223，从而抑制细胞的生长和侵袭，并诱导PC 细胞凋亡。我们发现金雀异黄素疗法明显抑制了 miR-223 的表达并上调了 Fbw7，miR- 223 的靶标之一。此外，MiR- 223的下调抑制了细胞生长并诱导 PC 细胞凋亡。这些结果表明，金雀异黄素部分通过下调 miR-223发挥其对PC细胞的抗肿瘤活性。