Tumor Biology, 2014, 35(11):11483-11488.

Apoptotic effect of genistein on human colon cancer cells via inhibiting the nuclear factor-kappa B (NF-κB) pathway

Yi Luo, Sheng-xiang Wang, Ze-quan Zhou, et al

Department of Health Management Center, Hangzhou Sanatorium of PLA, Hangzhou, People’s Republic of China; Department of Recuperation, Hangzhou Sanatorium of PLA, Hangzhou, China

Genistein possesses a wide variety of biological activities, and it is best known for its ability to inhibit cancer progression. Its cancer-preventive effect has been attributed to various mechanisms, including the induction of cell cycle arrest and apoptosis as well as the antioxidant functions. Nuclear factor kappa-B (NF-κB) is a signaling pathway that controls transcriptional activation of genes important for the tight regulation of many cellular processes and is aberrantly expressed in many types of cancer. Inhibitors of NF-κB pathway have shown potential anti-tumor activities. However, it is not fully elucidated in colon cancer. In the present study, we demonstrated that genistein could induce apoptosis in human colon cancer LoVo and HT-29 cells through inhibiting NF-κB pathway, as well as downregulation of Bcl-2 and upregulation of Bax, thus providing basis for clinical application of genistein in colon cancer cases.

英国《肿瘤生物学》，2014年11月

金雀异黄素通过抑制细胞核转录因子 κ B (NF-κ B) 途径对人体结肠癌细胞具有凋亡作用

罗伊、王胜祥、 周泽泉、 等

中国杭州中国人民解放军杭州疗养院健康管理中心和疗养部

金雀异黄素拥有广泛的生物活性，并且其中最出名的是它能够抑制癌症的进展。其预防癌症的效果已被归因于多种机制，包括诱导细胞周期阻滞和细胞凋亡，以及抗氧化功能。细胞核因子 κB (NF-κB) 是控制对于紧密调节许多细胞过程非常重要并且在许多癌症类型明显表达的基因的转录激活的信号通路。NF-κB 通路的抑制剂已被证明具有潜在的抗肿瘤活性。然而，其在结肠癌组织中没有被彻底阐明。在本研究中，我们证明了金雀异黄素通过抑制 NF-κB 通路，以及下调 Bcl-2 和上调 bax 表达，能够诱导人结肠癌 LoVo 细胞和HT-29 细胞凋亡，因而为金雀异黄素在结肠癌患者中的临床应用提供依据。