Human Cell, 2014, 27(2):78-84.

The effects of genistein and daidzein on cell proliferation kinetics in HT29 colon cancer cells: the expression of CTNNBIP1 (β-catenin), APC (adenomatous polyposis coli) and BIRC5 (survivin)

Sandra Regina Lepri, Leonardo Campos Zanelatto, et al

General Biology Department, State University of Londrina (UEL), Londrina, Brazil; Institute of BiosciencesSão Paulo State University, UNESP, Botucatu, Brazil

Soybean isoflavonoids have received significant attention due to their potential anticarcinogenic and antiproliferative effects and possible role in many signal transduction pathways. However, their mechanisms of action and their molecular targets remain to be further elucidated. In this paper, we demonstrated that two soybean isoflavones (genistein and daidzein) reduced the proliferation of the human colon adenocarcinoma grade II cell line (HT-29) at concentrations of 25 and 50-100 μM, respectively. We then investigated the effects of genistein and daidzein by RT-PCR on molecules that involved in tumor development and progression by their regulation of cell proliferation. At a concentration of 50 μM genistein, there was suppressed expression of β-catenin (CTNNBIP1). Neither genistein nor daidzein affected APC (adenomatous polyposis coli) or survivin (BIRC5) expression when cells were treated with concentrations of 10 or 50 μM. These data suggest that the down-regulation of β-catenin by genistein may constitute an important determinant of the suppression of HT-29 cell growth and may be exploited for the prevention and treatment of colon cancer.

日本《人类细胞》，2014年4月

金雀异黄素和大豆黄酮对结肠癌细胞HT29细胞增殖动力学的影响︰CTNNBIP1 (β-连环蛋白)，APC （大肠息肉症大肠杆菌） 和 BIRC5 （生存素）的表达

桑德拉  雷吉娜 雷普瑞，莱昂纳多  坎波斯 赞雷图，等

巴西隆德里纳国立大学普通生物学院；巴西圣保罗国立大学生物科学研究所

大豆异黄酮类化合物已经受到广泛的关注由于潜在的抗癌和抗增殖效果及其在许多信号转导通路中的可能作用。然而，它们的作用机制及其分子靶标仍有待进一步阐明。在本文中，我们证明了两种大豆异黄酮 （金雀异黄素和大豆黄酮） 分别在 25 和 50-100 uM浓度减少了人体结肠腺癌二级细胞 HT-29的增殖。然后我们通过逆转录-聚合酶链反应探究了金雀异黄素和大豆黄酮通过调节细胞增殖对参与肿瘤发生发展的分子的影响。当金雀异黄素的浓度为 50 μM时， β-连环蛋白 (CTNNBIP1) 的表达被抑制。当用 10 或 50 μ M 的浓度处理细胞时，金雀异黄素和大豆苷元都不会影响 APC （大肠息肉） 或生存素 (BIRC5) 的表达。这些数据表明金雀异黄素下调 β-catenin可能会成为抑制HT-29 细胞生长的重要决定因素，并且可能被用来预防和治疗结肠癌。