Molecular Nutrition & Food Research, 2015, 59(3):413-423.

Long-term exposure to dietary sources of genistein induces estrogen-independence in the human breast cancer (MCF-7) xenograft model

Juan E. Andrade, Young H. Ju, Chandra Baker, et al

Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, USA

SCOPE:

The long-term effect of exposure to relevant dietary levels of genistein (GEN) on estrogen receptor-positive (ER+) human breast cancer (MCF-7) progression after GEN withdrawal in athymic mice xenograft model was studied.

MATERIALS AND METHODS:

Feeding studies were conducted to determine the estrogenic effect of diets on MCF-7 tumor growth: (1) implantation (19 weeks) and withdrawal (6 weeks) of 17β-estradiol (E2 ); (2) dietary GEN 500 and 750 ppm during treatment/withdrawal for 23/10 and 15/9 weeks, respectively; and, (3) dietary soy protein isolate (SPI) containing GEN 180 ppm for 31/9 weeks of treatment/withdrawal. MCF-7 tumors grew fast in the presence of E2 implantation and abruptly regressed completely after E2 withdrawal. At different rates, dietary GEN alone (500 and 750 ppm) and GEN (180 ppm)-containing SPI stimulated MCF-7 tumor growth. After removal of the stimulus diet, tumors induced by 750 ppm GEN, but not 500 ppm GEN or SPI, regressed completely. The protein expression of epidermal growth factor receptor 2 (HER2) was higher in the GEN- and SPI-induced nonregressing (GINR) tumors compared to MCF-7 and E2 controls.

CONCLUSION:

Long-term consumption of low GEN doses (≤500 ppm) promotes MCF-7 tumor growth and results in GINR tumors with more aggressive and advanced growth phenotypes.

德国《分子营养与食品研究》，2014年3月

在人体乳腺癌 (MCF-7) 移植瘤模型中雌激素独立长期接触金雀异黄素的饮食源会诱导雌激素非依赖性生长

胡安 E 安德雷德，杨 H 居，钱德拉 贝克，等

美国伊利诺伊大学厄巴纳-香槟分校食品科学和人类营养学院

目的︰

研究接触相关膳食水平的金雀异黄素 （GEN）对撤回GEN以后的裸鼠移植瘤模型的雌激素受体阳性 （ER +） 人体乳腺癌 (MCF-7) 进展的长期影响。

材料和方法︰

进行了饲养研究以确定饮食对 MCF-7 肿瘤生长的雌激素影响: (1) 植入（19 周） 并撤出 （6 周） 的 17β-雌二醇 (E2);（2） 饮食GEN 500 和 750 ppm 用于治疗/撤销分别持续23/10 和 15 /9 周；而且，(3) 含有GEN 180 ppm的膳食大豆分离蛋白 (SPI)用于 31/9 周的治疗/撤出。当 E2 植入时MCF-7 肿瘤快速增长并且在E2 撤出后突然完全退化。以不同的速率，单独膳食GEN (500 和 750 ppm) 和含有GEN (180 ppm) 的SPI 刺激 MCF-7 肿瘤的生长。在除去刺激性饮食以后，肿瘤受到750 ppm GEN，而不是 500 ppm GEN或 SPI诱导完全退化。表皮生长因子受体 2 (HER2) 在GEN和 SPI 诱导的非退化型(GINR) 肿瘤中的蛋白质表达比MCF-7 和 E2 对照相比更高。

结论︰

低GEN剂量 (≤ 500 ppm) 的长期食用促进例如 MCF-7 肿瘤的生长并导致 GINR 肿瘤更具侵略性、更高级的增长表型。