Current Pharmaceutical Design, 2014, 20(33):5348-5353.

Genistein Inhibits Cell Growth and Invasion Through Regulation of miR-27a in Pancreatic Cancer Cells

Jun Xia, Long Cheng, Chuanzhong Mei, et al

Institute of Clinical Anatomy, Bengbu Medical College, Anhui, 233030, China

Abstract

BACKGROUND:

Although genistein has been reported to exert its anti-tumor activity, the exact mechanism of its action is poorly elucidated. Recently, it has been found that genistein could regulate the expression of microRNAs. Therefore, our aim in the present study was to find whether genistein regulates specific miR-27a in pancreatic cancer cells.

METHODS:

We performed our studies using multiple methods including MTT assay, RT-PCR, Western blotting analysis, migration, invasion assay, and transfection.

RESULTS:

We observed that genistein significantly inhibited the expression of miR-27a in pancreatic cancer cells. Moreover, inhibition of miR-27a suppressed cell growth and induced apoptosis as well as inhibited invasion in pancreatic cancer cells. Furthermore, we found a synergy effect between miR-27a and genistein on cell growth inhibition, apoptosis, and invasion, suggesting that targeting miR-27a may represent a potential strategy for treatment of pancreatic cancer.

CONCLUSIONS:

Our findings demonstrated that genistein plays a tumor suppressor role in part through inhibition of miR-27a in pancreatic cancer cells.

荷兰《当代制药设计》，2014年

在胰腺癌细胞中金雀异黄素通过调节miR-27a抑制细胞生长和侵袭

夏军，程龙，梅传忠，等

中国安徽蚌埠医学院临床解剖研究所

摘要

背景︰

虽然金雀异黄素被报道可以发挥抗肿瘤活性，其作用的确切机制尚不明确。最近，有人发现金雀异黄素可以调节小分子 RnNA的表达。因此，本研究的目的在于确认在胰腺癌细胞中金雀异黄素是否能调节特定的 miR-27a。

方法︰

我们使用包括 MTT 比色法测定，RT-PCR、 免疫蛋白印迹等多种方法研究细胞的迁移、 入侵和转染。

结果︰

我们观察到，金雀异黄素明显抑制了胰腺癌细胞中miR-27a的表达。此外，miR-27a的抑制又抑制了细胞的生长并诱导细胞凋亡以及抑制胰腺癌细胞的侵袭。此外，我们发现 miR-27a 和金雀异黄素之间的协同效应对细胞生长抑制、 凋亡及入侵，表明靶向miR-27a 可能是一种治疗胰腺癌潜在的方法。

结论︰

我们的研究结果表明，在胰腺癌细胞中金雀异黄素部分通过miR-27a的抑制作用发挥抑制癌症的作用。