Climacteric the Journal of the International Menopause Society, 2014, 18(3):389-98.

Soybean isoflavones attenuate the expression of genes related to endometrial cancer risk

M Aparecida Santos, et al

Department of Morphology and Genetics, University Federal of São Paulo; et al

OBJECTIVE:

We evaluated whether genistein or estrogen treatment has the same effect when administered immediately or late to rats induced with menopause using ovariectomy.

METHODS:

Sixty adult female rats were divided into six treatment groups: GI = vehicle immediately after ovariectomy; GII = vehicle 30 days after ovariectomy; GIII = genistein immediately after ovariectomy; GIV = genistein 30 days after ovariectomy; GV = estrogen immediately after ovariectomy; and GVI = estrogen 30 days after ovariectomy. All animals were treated for 30 consecutive days. At the end of the treatment, part of the uteri was removed for subsequent histological studies and another part was used to evaluate estrogen receptors 1 and 2, cell proliferation (cyclin A1 and A2, cyclin D1, cyclin-dependent kinase inhibitors 1, 1B and 2, antigen identified by the monoclonal antibody Ki67) and angiogenesis (vascular endothelial growth factor, VEGF-A) gene expression.

RESULTS:

Late treatment after castration in rats resulted in more developed endometrium, enhanced cell proliferation and estrogen-signalling pathways, particularly the cyclin-related genes Ki67 and VEGF-A, compared to early treatment. Interestingly, these same effects were less intense with genistein compared to those induced by estrogen, especially when genistein was administered late.

CONCLUSION:

Our data show that isoflavone renders a lower risk of cancer when compared to estrogen in treatments.

英国《更年期：国际更年期学会学报》，2014年6月

大豆异黄酮减弱与子宫内膜癌风险有关基因的表达

M 阿帕雷西达 桑托斯，等

圣保罗联邦大学形态学和遗传学学院等

目的︰

当对使用了卵巢切除术的绝经期大鼠立即给药或延迟给药时，我们评估了金雀异黄素或雌激素治疗是否具有相同的效果。

方法︰

60只成年雌性大鼠被分为六个治疗小组︰ GI = 卵巢切除后即时空白样；GII =卵巢切除后30 天的空白样；GIII =卵巢切除后即时的金雀异黄素；GIV = 卵巢切除30 天后的金雀异黄素；GV =卵巢切除后即时的雌激素；  GVI =卵巢切除30 天后的雌激素。所有动物均连续治疗30 天。在治疗结束时，部份子宫体被切除用于随后的组织学研究，另一部分则用于评估雌激素受体 1 和 2，细胞增殖（细胞周期蛋白 A1 和 A2，细胞周期蛋白 D1、 细胞周期蛋白依赖性激酶抑制剂 1、 1B 和 2，单克隆抗体 Ki67的抗原）和血管生成 (血管内皮生长因子，VEGF-A) 基因的表达。

结果︰

与早期治疗相比，大鼠去势后的延迟治疗导致子宫内膜的进展，增强的细胞增殖和雌激素信号通路，特别是细胞周期相关基因 Ki67 和 VEGF-A。有趣的是，金雀异黄素的效果不像雌激素诱导那样强烈，尤其是当金雀异黄素被延迟给药时。

结论︰