Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2015, 84:64-73.

Genistein reduces the noxious effects of in utero bisphenol A exposure on the rat prostate gland at weaning and in adulthood

Bruna Dias Bernardo, Joyce Zalotti Brandt, et al

Univ. Estadual Paulista, School of Medicine, Department of Pathology, Botucatu, SP, 18618-970, Brazil; Univ. Estadual Paulista, Institute of Biosciences, Department of Morphology, Botucatu, SP, 18618-970, Brazil

Bisphenol A (BPA) is one hormonally active chemical with potential deleterious effects on reproductive organs, including breast and prostate. In contrast, genistein (GEN) is the major phytoestrogen of soy that presents potential protective effects against hormone-dependent cancers, including that of the prostate. Thus, pregnant Sprague–Dawley rats were treated with BPA at 25 or 250 μg/kg/day by gavage from gestational day (GD) 10–21 with or without dietary GEN at 250 mg/kg/chow (∼5.5 mg/kg/day). Then, male offspring from different litters were euthanized on post-natal day (PND) 21 and 180. At PND21, BPA 25 exposure induced early prostatic changes while dietary GEN attenuated some deleterious actions this xenoestrogen on epithelial cell proliferation levels, androgen receptor expression and prostatic architecture in male offspring. At PND180, a significant increase in incidence of prostatic multifocal inflammation/reactive hyperplasia and atypical hyperplasia were observed in male offspring from dams that received BPA 25. On the other hand, maternal GEN feeding attenuated some the adverse effects of BPA 25 on prostate disease at late-in-life. This way, the present findings point to preventive action of dietary GEN on deleterious effects of gestational BPA exposure in both early and late prostate development in offspring.

英国《食品和化学毒理学》，2015年10月

金雀异黄素降低了子宫内双酚A在断奶和成年时接触大鼠前列腺腺体的毒性作用

布鲁纳 迪亚斯 贝尔纳多，乔伊斯 泽劳提 布兰德，等

巴西圣保罗州立大学医学院病理学系以及生物科学研究所形态学系

双酚A（BPA）是一种激素活性化合物，对生殖器官，包括乳腺和前列腺具有潜在的有害影响。 相比之下，金雀异黄素（GEN）是大豆的主要植物雌激素，对激素依赖性癌症包括前列腺癌具有潜在的保护作用。 因此，按照妊娠日（GD）为10-21天的管饲法用25或250μg/ kg /天的BPA处理怀孕的Sprague-Dawley大鼠，其中含有或不含有250mg / kg /剂量的金雀异黄素（~5.5mg / kg / 天）。 然后，在产后（PND）第21日和180日，将不同组的雄性后代安乐死。在PND21，BPA 25暴露引起早期前列腺变化，而膳食GEN降低了这种异种雌激素对上皮细胞的增殖水平，雄激素受体的表达和 男性后代前列腺的发育。在PND180，在接受BPA 25的雄性大鼠后代中观察到前列腺多灶性炎症/反应性增生和非典型增生的发病率显着增加。另一方面，母体GEN进食减少了BPA 25对前列腺疾病的一些不利影响后期的生活。 这样，目前的研究结果表明饮食GEN接触妊娠BPA对后代早期和晚期前列腺发育的有害影响具有预防作用。