Reproductive Toxicology, 2015, 54:101-109.

Dietary exposure in utero and during lactation to a mixture of genistein and an anti-androgen fungicide in a rat mammary carcinogenesis model

J. P. Brouland, H. EI Sheikh Saad, et al

Centre des Sciences du Gout et de l'Alimentation, F-21000 Dijon, France; Université de Bourgogne, Dijon, France; et al

Endocrine disruptors may play substantial roles in the high incidence of breast cancer. We previously described how early exposure to the mixture of phytoestrogen genistein (G) and the anti-androgen vinclozolin (V) affects peripubertal mammary development. This study evaluates the carcinogenic potential of exposure to V alone or associated with G from conception until weaning in Wistar rats. Dams were exposed to V, G or GV during pregnancy/lactation. At PND50 offspring were treated with DMBA[7,12-dimethylbenz(a)anthracene]. V or GV maternal exposure decreased number of DMBA-induced mammary tumors in the offspring, without significant modifications in tumor incidence, multiplicity and latency. G exposure decreased number of tumors, incidence and multiplicity. Unexpectedly, GV exposure increased tumor volume (p=0.04 vs controls) and epithelial proliferation (p=0.001 vs controls; p=0.005 vs G,V only). All tumors were in situ carcinomas. Concluding, maternal gestation/lactation exposure to a vinclozolin and genistein mixture significantly increases offspring tumor growth without changes in carcinogenesis susceptibility.

美国《生殖毒理学》，2015年7月

在乳腺癌大鼠模型中哺乳期子宫和金雀异黄素与抗雄激素杀菌剂混合物的接触

J.P.布鲁朗，H姆沙伊赫萨阿德，等

法国勃艮第大学科学中心味道和营养学院等

内分泌干扰物对于乳腺癌的高发生率可能发挥重大作用。我们前面研究了植物性雌激素金雀异黄素(G) 与抗雄激素乙烯菌核利 (V)的混合物的早期接触如何影响青春期乳房的发育。本研究旨在评估从受孕至断奶Wistar 大鼠单独接触 V或与 G 一起的致癌潜力。在怀孕/哺乳期接触V、 G 或 GV。用DMBA [7，12-二甲基苯并 (a) 蒽]处理 PND50 的后代。V 或 GV 母系的接触减少了 后代DMBA 诱导的乳腺肿瘤的数量，而在肿瘤的发病率、 多样性和潜伏期等没有明显变化。G 的接触降低了肿瘤的数量，发病率和多样性。出乎意料的是，GV 接触增加了肿瘤体积 (p = 0.04 vs 对照组) 和上皮细胞的增殖 (p = 0.001 vs 对照组; p = 0.005 vs G，V 单独)。所有肿瘤都是原位肿瘤。结论，母系妊娠期/哺乳期接触乙烯菌核利和金雀异黄素的混合物大大明显提高了后代肿瘤的生长而癌变过程中的易感性没有变化。