Molecular Nutrition & Food Research, 2015, 59(8):1419–1430.

Isoflavones in soy flour diet have different effects on whole-genome expression patterns than purified isoflavone mix in human MCF-7 breast tumors in ovariectomized athymic nude mice

Yunxian Liu, Leena Hilakivi-Clarke, Yukun Zhang, et al

Department of Food Science and Human Nutrition, University of Illinois, Urbana-Champaign, IL, USA; et al

SCOPE:

Soy flour diet (MS) prevented isoflavones from stimulating MCF-7 tumor growth in athymic nude mice, indicating that other bioactive compounds in soy can negate the estrogenic properties of isoflavones. The underlying signal transduction pathways to explain the protective effects of soy flour consumption were studied here.

METHODS AND RESULTS:

Ovariectomized athymic nude mice inoculated with MCF-7 human breast cancer cells were fed either Soy flour diet (MS) or purified isoflavone mix diet (MI), both with equivalent amounts of genistein. Positive controls received estradiol pellets and negative controls received sham pellets. GeneChip Human Genome U133 Plus 2.0 Array platform was used to evaluate gene expressions, and results were analyzed using bioinformatics approaches. Tumors in MS-fed mice exhibited higher expression of tumor growth suppressing genes ATP2A3 and BLNK and lower expression of oncogene MYC. Tumors in MI-fed mice expressed a higher level of oncogene MYB and a lower level of MHC-I and MHC-II, allowing tumor cells to escape immunosurveillance. MS-induced gene expression alterations were predictive of prolonged survival among estrogen-receptor-positive breast cancer patients, whilst MI-induced gene changes were predictive of shortened survival.

CONCLUSION:

Our findings suggest that dietary soy flour affects gene expression differently than purified isoflavones, which may explain why soy foods prevent isoflavones-induced stimulation of MCF-7 tumor growth in athymic nude mice.

德国《分子营养与食品研究》，2015年8月

在患有MCF-7乳腺肿瘤并切除卵巢的裸鼠中大豆粉状食物中的异黄酮比纯化的大豆异黄酮组合物对全基因组表达模式有更加与众不同的影响

刘云仙，丽娜 希拉基维-克拉克，张玉坤，等

美国伊利诺伊大学厄巴纳-尚佩恩分校食品科学和人类营养学院等

目标︰

大豆粉状膳食 (MS) 阻止了异黄酮对无胸腺裸鼠中MCF-7 肿瘤生长的刺激，表明其他的大豆生物活性化合物可以抵消异异黄酮的雌激素属性。这里研究了基础信号转导通路来解释大豆粉状膳食的保护作用。

方法和结果︰

接种了 MCF-7 人体乳腺癌细胞 (MS)的去势裸鼠被喂养大豆粉状饮食或纯化的异黄酮混合饲料 (MI)，二者具有相同含量的金雀异黄素。阳性对照组接受雌二醇颗粒而阴性对照组接受空的微丸。基因芯片人体基因组 U133 和 2.0阵列平台被用于评估基因的表达，并且利用生物信息学方法对结果进行分析。MS 喂养小鼠的肿瘤生长抑制基因 ATP2A3 和BLNK表达更高，而低癌基因MYC表达更低。MI 喂养小鼠的癌基因 MYB 表达更高和 MHC-I和 MHC-II的水平更低，使肿瘤细胞摆脱免疫监视。预测MS 诱导基因表达的改变可以延长雌激素受体阳性乳腺癌患者的生存时间，而预测 MI 诱导基因的变化是缩短生存期。