Oncology Letters, 2016, 12(3).

Genistein inhibits A549 human lung cancer cell proliferation via miR-27a and MET signaling

Yang Yang, Aimin Zang, Youchao Jia, et al

Department of Medical Oncology, Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China

Genistein is a soybean isoflavone; in its aglycone it has various biological activities. Animal experiments, clinical studies and epidemiological investigations suggest that genistein has preventative and curative functions for a number of diseases, particularly in cancer. The present study explored the potential anti-cancer effect of genistein by observing its role in inhibiting A549 human lung cancer cell proliferation and investigating the possible mechanism. A549 cells were exposed to various concentrations of genistein (0, 10, 25, 50, 100 and 200 µM; dissolved in physiological saline) for 1, 2 and 3 days. Subsequently, the viability of A549 cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell apoptosis was examined using a flow cytometer, caspase 3/9 activity was measured using commercial kits, reverse transcription quantitative polymerase chain reaction was used to analyze the miR-27a expression and western blotting was used to investigate MET protein expression. The results suggested a significant inhibition of A549 cell growth following treatment with genistein in a time- and dose-dependent manner. The current study also indicated that treatment with genistein significantly induces cell apoptosis and promotes caspase-3/9 activation of A549 cells in a dose-dependent manner. Further functional assays revealed that the anti-cancer effect of genistein activated microRNA-27a (miR-27a) expression levels and reduced MET protein expression in A549 cells. In conclusion, the present study demonstrates that genistein inhibits A549 human lung cancer cell proliferation. Furthermore, this study reports, for the first time, a correlation between the anti-cancer effect of genistein and miR-27a-mediated MET signaling.

中国《肿瘤学》, 2016, 12(3).

染料木黄酮通过miR-27a 和MET信号抑制 A549 人体肺癌细胞的增殖

杨阳，臧爱民，贾友超等

河北大学附属医院肿瘤放射治疗化学治疗河北省重点实验室肿瘤科，河北保定071000

染料木黄酮是一种大豆异黄酮，在其糖苷配基中具有各种生物活性。动物实验，临床研究和流行病学调查表明染料木素对许多疾病，特别是癌症具有预防和治疗功能。本研究通过观察其在抑制A549人肺癌细胞增殖中的作用，探讨染料木素的潜在抗癌作用，探讨其可能的机制。将A549细胞暴露于各种浓度的染料木黄酮（0,10,25,50,100和200μM;溶解于生理盐水中）1,2和3天。随后，通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物测定确定A549细胞的存活力，使用流式细胞仪检查细胞凋亡，使用商业试剂盒，逆转录定量聚合酶链反应用于分析miR-27a表达，并使用western印迹来研究MET蛋白表达。结果表明，用染料木黄酮以时间和剂量依赖的方式处理后，A549细胞生长的显着抑制。目前的研究还表明，用染料木黄酮处理显着诱导细胞凋亡，并以剂量​​依赖的方式促进A549细胞的半胱天冬酶-3 / 9激活。进一步的功能测定揭示了染料木黄酮激活微小RNA-27a（miR-27a）的表达水平和减少MET蛋白表达在A549细胞中的抗癌作用。总之，本研究表明染料木黄酮抑制A549人肺癌细胞增殖。此外，这项研究首次报告了染料木黄酮和miR-27a介导的MET信号传导的抗癌作用之间的相关性。