European Review for Medical & Pharmacological Sciences, 2016, 20(15).

Genistein-induced apoptosis is mediated by endoplasmic reticulum stress in cervical cancer cells

Y-M Yang, Y Yang, W-W Dai, et al

Cancer Research Institute, Harbin Medical University, Harbin, China

OBJECTIVE:

Genistein, a major isoflavone found in soybeans, exhibits anti-cancer activity. Endoplasmic reticulum (ER) stress is known to be implicated in apoptosis induced by anti-cancer drugs. This study aimed to characterize the role of ER stress in genistein-induced apoptosis in cervical cancer.

MATERIALS AND METHODS:

HeLa cells were treated with genistein or/and 4-phenylbutyric acid. Cell viability and apoptosis were evaluated by MTT assay and flow cytometry. Protein levels were detected by Western blot analysis.

RESULTS:

Genistein suppressed the viability of HeLa cells in a dose dependent manner. In addition, genistein caused apoptosis in HeLa cells in a dose dependent manner. Genistein triggered ER stress in HeLa cells, as indicated by the upregulation of glucose-regulated protein 78 (GRP78) and CHOP expression. Furthermore, ER stress inhibitor 4-phenylbutyric acid alleviated genistein-induced apoptosis and ER stress in HeLa cells.

CONCLUSIONS:

Our results suggest that ER stress contributes to genistein-induced apoptosis in cervical cancer cells, and genistein is a promising agent for cervical cancer therapy.

罗马《欧洲评论医疗及药理科学》, 2016, 20(15).

染料木黄酮诱导的细胞凋亡是由宫颈癌细胞中的内质网应激介导的

Y-M Yang, Y Yang, W-W Dai, et al

哈尔滨医科大学肿瘤研究所，哈尔滨

目的：

染料木黄酮是在大豆中发现的主要异黄酮，表现出抗癌活性。已知内质网（ER）应激牵涉由抗癌药物诱导的细胞凋亡。本研究旨在表征ER应力在染料木黄酮诱导的宫颈癌细胞凋亡中的作用。

材料和方法：

用染料木黄酮或/和4-苯基丁酸处理HeLa细胞。通过MTT测定和流式细胞术评价细胞存活力和凋亡。通过蛋白质印迹分析检测蛋白质水平。

结果：

染料木黄酮以剂量依赖性方式抑制HeLa细胞的生存力。此外，染料木黄酮以剂量依赖性方式在HeLa细胞中引起凋亡。 Genistein触发ER应力在HeLa细胞，如葡萄糖调节蛋白78（GRP78）和CHOP表达的上调所示。此外，ER应力抑制剂4-苯基丁酸减轻染料木黄酮诱导凋亡和ER应力在HeLa细胞。

结论：

我们的研究结果表明ER应力有助于染料木黄酮诱导宫颈癌细胞凋亡，染料木素是一种有前景的宫颈癌治疗药物。