J Cancer Prev Curr Res 2016, 4(2): 000111.

Enhancement of Genistein-Induced Apoptosis in LNCaP Prostate Cancer cells

Toluleke Famuyiwa, Joubin Jebelli

Florida Atlantic University

This study determined the impact of vitamin C dosage on genistein-induced apoptosis in LNCaP cancer cells in vitro at various treatment regimens. Although the linear regression of viability assay (MTT) indicated a p-value = 0.11; NBT assay reveal a declining SOD activity during cell death. Apoptosis induction was the main mode of treatment induced cell death. The overall data showed the trend of treatment efficacy as; (Gen 10uM + Vit C 40uM) > (Gen 30uM + Vit C 40uM) > (Gen 70uM + Vit C 40uM) >10uM genistein > 70uM genistein. The chi-square test for comparing necrosis, apoptosis, and live cells showed that Vitamin C could impact genistein-induced apoptosis in LNCaP cells (p = 0.0003). This study forms basis for in vivo studies of the impact of vitamin C on genistein-induced apoptosis in LNCaP prostate cancer cells.

《当前癌症预防杂志》2016, 4(2): 000111.

金雀异黄素诱导并增强前列腺癌症细胞LNCaP的凋亡

佛罗里达大西洋大学

这项研究确定维生素C剂量对染料木素诱导的凋亡在LNCaP癌细胞体外各种治疗方案的影响。虽然生存力测定（MTT）的线性回归表明p值= 0.11; NBT测定显示细胞死亡期间SOD活性下降。凋亡诱导是治疗诱导的细胞死亡的主要模式。总体数据显示治疗效果的趋势; （Gen10uM+VC40uM）>（Gen30uM+VC40uM）>（Gen70uM + VC40uM）>10uM染料木黄酮>70uM染料木黄酮。 用于比较坏死，凋亡和活细胞的卡方检验显示维生素C可以影响LNCaP细胞中染料木黄酮诱导的凋亡（p = 0.0003）。这项研究形成基因的体内研究维生素C对染料木素诱导细胞凋亡在LNCaP前列腺癌细胞的影响。