ORIENTAL JOURNAL OF CHEMISTRY, 2016, 32(3), 1619-1624.

The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein-Standardized Ethanolic Extract of Tempeh - A Fermented Product of Soybean (Glycine max)

Sri Hartati Yuliani, Enade Perdana Istyastono, Florentinus Dika Octa Riswanto

Faculty of Pharmacy, Universitas Sanata Dharma, Campus 3 Paingan, Maguwohardjo, Depok, Sleman Yogyakarta 55282, Indonesia

As fermented soybean (Glycine max), tempeh has been reported as a good source of isoflavones, which have shown anticancer activities in various cancer cells resulted from their activity as phytoestrogens. In this article, the preparation of ethanolic extract of tempeh is presented and then followed by the exploration of its cytotoxic effect on T47D cells. The extract was subsequently standardized using genistein, the major phytoestrogen found in tempeh. The genistein concentration found in the extract of tempeh was 0.681 % (w/w). The cytotoxicity assay employing 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) resulted in IC50 values of 196.066 ±15.956 µg/mL and 13.174 ± 0.905 µg/mL for the genistein-standardized ethanolic extract of tempeh and genistein, respectively.

《东方化学杂志》2016

豆豉，一种大豆的发酵产物，其金雀异黄素标准的乙醇提取物对T47D乳腺癌细胞的毒害活性

药学院，大学Sanata Dharma，Kampus 3 Paingan，Maguwoharjo，Depok，Sleman Yogyakarta 55282，印度尼西亚

作为发酵大豆（大豆），豆豉已被报道为异黄酮的良好来源，其在各种癌细胞中显示出抗癌活性，这是由于它们作为植物雌激素的活性。 在本文中，提出了豆豉乙醇提取物的制备，然后探讨其对T47D细胞的细胞毒性作用。随后使用染料木黄酮标记提取物，染料木黄酮是在豆豉中发现的主要植物雌激素。 在豆豉的提取物中发现的染料木黄酮浓度为0.681％（w/w）。使用3-[4,5-二甲基噻唑-2-基] -2,5-二苯基四唑溴化物（MTT）的细胞毒性测定导致对于染料木黄酮-2的四环素的IC 50值为196.066±15.956μg/ mL和13.174±0.905μg/ 分别是豆豉和染料木素的标准化乙醇提取物。