Journal of Experimental & Clinical Cancer Research Cr, 2009, 28(1):1-7.

Effect of Genistein on vasculogenic mimicry formation by human uveal melanoma cells

Rihong Cong,Qingmin Sun, et al

1Department of Pharmacology, Nanjing Medical University, Nanjing 210029, PR China and 2Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China

BACKGROUND:

Vasculogenic mimicry (VM) was increasingly recognized as a form of aggressive melanoma acquiring blood supply. Genistein had attracted much attention as a potential anticancer agent. Therefore, we examined the effect of Genistein on VM in human uveal melanoma cells.

METHODS:

VM structure was detected by periodic acid-Schiff (PAS) staining for uveal melanoma C918 cells cultured on the three-dimensional type I collagen gels after exposed to Genistein. We used reverse transcription polymerase chain reaction (RT-PCR) and Western Blot analysis to examine the effect of Genistein on vascular endothelial cadherin (VE-cadherin) mRNA and protein expression. The nude mice models of human uveal melanoma C918 cells were established to assess the number of VM using immunohistochemical and PAS double-staining.

RESULTS:

Genistein inhibited the survival of C918 cells in vitro. The ectopic model study showed that VM in tumor tissue sections were significantly reduced by Genistein in vivo. In vitro, the VM structure was found in control, 25 and 50 microM Genistein-treatment groups but not in 100 and 200 microM. RT-PCR and Western Blot showed that 100 and 200 microM concentration of Genistein could significantly decrease VE-cadherin mRNA and protein expression of C918 cells compared with control (P < 0.05). However, the 25 and 50 microM Genistein slightly decreased the VE-cadherin level in vitro (P > 0.05).

CONCLUSION:

Genistein inhibits VM formation of uveal melanoma cells in vivo and in vitro. One possible underlying molecular mechanism by which Genistein could inhibit VM formation of uveal melanoma is related to down-regulation of VE-cadherin.

英国《实验与临床癌症研究杂志》，2009年

金雀异黄素对模拟人葡萄膜黑素瘤细胞的血管形成的影响

丛日红，孙清敏等

南京医科大学药理学教研室南京210029;南京医科大学第一附属医院普外外科南京210029

背景：

血管生成模拟（VM）越来越多地被认为是侵略性黑色素瘤获得血液供应的一种形式。染料木黄酮作为潜在的抗癌药物引起了广泛关注。因此，我们检查了染料木黄酮对人类葡萄膜黑素瘤细胞中VM的影响。

方法：

通过在暴露于染料木黄酮后在三维I型胶原凝胶上培养的葡萄膜黑素瘤C918细胞，通过定期酸 - 希夫（PAS）染色检测VM结构。我们使用逆转录聚合酶链反应（RT-PCR）和Western Blot分析来检测染料木黄酮对血管内皮钙粘蛋白（VE-cadherin）mRNA和蛋白表达的影响。建立了人类葡萄膜黑色素瘤C918细胞的裸鼠模型，用免疫组织化学和PAS双染法评估VM的数量。

结果：

染料木素在体外抑制C918细胞的存活。异位模型研究显示，染色木质素在体内的肿瘤组织切片中的VM显着降低。在体外，VM结构发现在对照，25和50 microM染料木黄酮治疗组，但不是在100和200 microM。 RT-PCR和Western Blot显示，与对照组相比，100和200 microM浓度的染料木黄酮可显着降低C918细胞的VE-钙粘蛋白mRNA和蛋白表达（P <0.05）。然而，25和50 microM染料木黄酮体外VE-钙粘蛋白水平略有降低（P> 0.05）。

结论：

染料木黄酮体内和体外抑制葡萄膜黑素瘤细胞的VM形成。染料木黄酮可以抑制葡萄膜黑素瘤VM形成的一个潜在的分子机制与VE-钙粘蛋白的下调有关。