World Journal of Gastroenterology, 2009, 15(39):4952-4957.

Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma

Yan Gu,Cheng-Fang Zhu, et al

Department of General Surgery, Shanghai Ninth Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; et al

 

AIM: To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism.

METHODS: MHCC97-H hepatocellular carcinoma cells were exposed to genistein. A cell attachment assay was carried out in a microculture well pre-coated with fibronectin. The invasive activity of tumor cells was assayed in a transwell cell culture chamber, and cell cycle and apoptosis were evaluated by a functional assay. In addition, the expression and phosphorylation of FAK were detected by Western blotting. In situ xenograft transplantation of hepatocellular carcinoma was performed in 12 nude mice and lung metastasis of hepatocellular carcinoma was observed.

RESULTS: Genistein significantly inhibited the growth of MHCC97-H cells in vitro. Adhesion and invasiveness of MHCC97-H cells were inhibited in a concentration-dependent fashion, and the inhibitory effect of genistein was more potent in the 10 μg/mL and 20 μg/mL genistein-treated groups. Genistein caused G0/G1 cell cycle arrest, an S phase decrease, and increased apoptosis. The expression and phosphorylation of FAK in MHCC-97H cells were significantly decreased. In situ xenograft transplantation of hepatocellular carcinoma was also significantly suppressed by genistein. The number of pulmonary micrometastatic foci in the genistein group was significantly lower compared with the control group (12.3 ± 1.8 vs 16.6 ± 2.6, P < 0.05).

CONCLUSION: Genistein appears to be a promising agent in the inhibition of metastasis of hepatocellular carcinoma.

中国《世界胃肠病学杂志》，2009年

金雀异黄素对人肝细胞癌转移的抑制作用

严古，朱成芳，等

上海交通大学医学院上海第九医院普外外科，上海200011; 等

 

目的：调查染料木黄酮对MHCC97-H肝细胞癌细胞转移的抑制作用，探讨其潜在的机制。

方法：将MHCC97-H肝细胞癌细胞暴露于染料木黄酮。在微量培养皿中进行细胞附着测定预先涂布纤连蛋白。在transwell细胞培养室中测定肿瘤细胞的侵袭活性，细胞周期和细胞凋亡通过功能测定进行评估。此外，通过Western印迹检测FAK的表达和磷酸化。原位在12只裸鼠中进行肝细胞癌异种移植，肝细胞癌肺转移为观察到的。

结果：染料木黄酮体外显着抑制MHCC97-H细胞的生长。抑制MHCC97-H细胞的粘附和侵袭以浓度依赖的方式，染料木素的抑制作用在10μg/ mL和20μg/ mL染料木黄酮治疗中更有效组。染料木黄酮引起G0 / G1细胞周期停滞，S期下降，凋亡增加。 FAK的表达和磷酸化MHCC-97H细胞明显减少。原位异种移植肝细胞癌也被显着抑制金雀异黄素。染料木素组肺部微转移灶数显着低于对照组（12.3％）±1.8 vs 16.6±2.6，P <0.05）。

结论：金雀异黄素似乎是抑制肝细胞癌转移的有前途的药物。