Clinical Cancer Research An Official Journal of the American Association for Cancer Research, 2000, 6(1):230.

The potential of soybean foods as a chemoprevention approach for human urinary tract cancer

Shu-Jem Su, Huan-Yao Lei, et al

Departments of Microbiology and Immunology Medical Technology and Pathology College of Medicine, National Cheng Kung University, Tainan,Taiwan 70101, Republic of China

Isoflavones are excreted in human urine and can be modulated by soy-rich diets. Recently, isoflavones were suggested to have protective effects against bladder cancer cells. We sought todetermine the efficacy of the antitumorigenic effects of isoflavones at concentrations found in the range of human urine excretion and compare normal urothelium and bladder cancer cells for differential cytotoxicity. A total of seven human bladder cancer cell lines and an immortalized uroepithelial cell line were used to examine the effects of genistein, daidzein, and biochanin-A, either individually or as an equal-proportion mixture regimen, on cell growth, DNA synthesis, alterations of cell cycle distribution,  and induction of apoptosis. The role of cyclin B1 and cdc2 kinase in cell cycle arrest was analyzed. In addition, severe combined immunodeficient mice were used to confirm the anti-cancer effects of isoflavones in vivo. Cooperative action of isoflavones was more effective in growth inhibition and apoptosis induction than any single compound. Genistein tends to cause a dose-dependent induction of G2-M cell cycle arrest and an inhibition of cdc2 kinase activity. However, both daidzein and biochanin-A directly induced apoptosis without altering cell cycle distribution. The IC50 values in non-transformed cells were higher than those in most cancer cell lines, and the IC50 of the mixture regimen was within reach of the levels observed in urine after a soy challenge. Furthermore, both genistein and combined isoflavones exhibited a significant tumor suppressor effect in vivo (P < 0.05). The results justify the potential use of soybean foods as a practical chemoprevention approach for patients with urinary tract cancer.

美国《临床癌症研究美国癌症研究协会官方杂志》

大豆食品作为人类尿路癌化学预防方法的潜力

苏书杰，雷焕耀，等

国立成功大学医学技术与病理学院微生物与免疫学系，台南，台湾70101，中华民国

异黄酮在人尿液中排泄，可以用大豆丰富的饮食调节。最近，异黄酮被认为有保护作用对膀胱癌细胞的作用。我们试图确定异黄酮在发现浓度下的抗肿瘤作用的功效在人尿排泄范围内，比较正常的尿路上皮和膀胱癌细胞的差异细胞毒性。共七个人膀​​胱癌细胞系和永生化尿路上皮细胞系用于检测染料木素，黄豆苷元和单纯地或作为等比例的混合物方案对细胞生长，DNA合成，细胞周期分布的改变，和诱导凋亡。分析细胞周期蛋白B1和cdc2激酶在细胞周期停滞中的作用。另外，严重结合免疫缺陷小鼠用于确认异黄酮在体内的抗癌作用。异黄酮的合作作用更为有效在生长抑制和凋亡诱导中比任何单一化合物。染料木黄酮倾向于引起G2-M细胞的剂量依赖性诱导周期停滞和cdc2激酶活性的抑制。然而，黄豆苷原和鹰嘴豆素-A都直接诱导凋亡而不改变细胞循环分布。非转化细胞中的IC 50值高于大多数癌细胞系中的IC50值，混合物的IC50值在大豆攻击后尿液中观察到的水平达到了程度。此外，染料木素和组合的异黄酮均呈现出来体内显着的肿瘤抑制作用（P <0.05）。结果证明大豆食品作为实际化学预防的潜在用途是对尿路癌患者来说是合理的方法。