World Journal of Gastroenterology, 2005, 11(1):69-72.
Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma
HongBin Cui, XiaoLin Na, DanFeng Song, et al
Department of Food Nutrition and Hygiene, Public Health College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
AIM: To study the blocking effects of genistein on cell proliferation cycle in human gastric carcinoma cells (SGC-7901) and the possible mechanism.
METHODS: MTT assay was applied in the detection of the inhibitory effects of genistein on cell proliferation. Flow cytometry was used to analyze the cell cycle distribution. Immunocytochemical technique and Western blotting were performed to detect the protein expression of cyclin D1, cyclin B1 and p21waf1/cip1.
RESULTS: Genistein significantly inhibited the growth and proliferation of human gastric carcinoma cells (SGC-7901). Seven days after treatment with different concentrations of genistein (2.5, 5.0, 10.0, 20.0 μg /mL), the growth inhibitory rates were 11.2%, 28.8%, 55.3%, 84.7% respectively and cell cycles were arrested at the G(2)/ M phase. Genistein decreased cyclin D1 protein expression and enhanced cyclin B1 and p21waf/cip1 protein expression in a concentration-dependent manner.
CONCLUSION: The growth and proliferation of SGC-7901 cells can be inhibited by genistein via blocking the cell cycle, with reduced expression of cyclin D1 and enhanced expression of cyclin B1 and p21waf/cip1 protein in the concentration range of 0-20 μg /mL.
中国《世界胃肠病学杂志》，2005年
金雀异黄素对人胃癌细胞增殖发挥阻断作用的可能机制
崔宏斌，那小林，宋丹凤，等
哈尔滨医科大学公共卫生学院食品营养与卫生系，哈尔滨，黑龙江省
目的：研究染料木黄酮对人胃癌细胞（SGC-7901）细胞增殖循环的阻断作用及其机制。
方法：应用MTT法检测染料木黄酮对细胞增殖的抑制作用。流式细胞术用于分析细胞周期分布。进行免疫细胞化学技术和Western印迹检测细胞周期蛋白D1，细胞周期蛋白B1和p21waf1 / cip1的蛋白表达。
结果：染料木黄酮显着抑制人胃癌细胞（SGC-7901）的生长和增殖。用不同浓度的染料木黄酮（2.5,5.0,10.0,20.0μg/ mL）处理7天，生长抑制率分别为11.2％，28.8％，55.3％，84.7％，细胞周期在G（2） / M相。染料木黄酮以浓度依赖性方式降低细胞周期蛋白D1蛋白表达，增强细胞周期蛋白B1和p21waf / cip1蛋白表达。
结论：SGC-7901细胞的生长和增殖可通过阻断细胞周期抑制细胞周期蛋白的表达，细胞周期蛋白D1的表达降低，细胞周期蛋白B1和p21waf / cip1蛋白的表达浓度范围为0〜20μg/ mL 。
