Journal of Nutrition, 1995, 125(3Suppl):777S-783S.

Effect of genistein on in vitro and in vivo models of cancer

Stephen Barnes

Department of Pharmacology, University of Alabama at Birmingham 35294

In two-thirds of studies on the effect of genistein-containing soy materials in animal models of cancer, the risk of cancer (incidence, latency or tumor number) was significantly reduced. In addition, purified genistein delayed mammary tumor appearance in association with increased cell differentiation in mammary tissue in rats treated with 7, 12-dimethylbenz[a]anthracene when administered neonatally, inhibited phorbol ester-induced H2O2 production in a model of skin cancer, and inhibited aberrant crypt formation in a model of colonic cancer. In in vitro models, 

genistein inhibited the proliferation of human tumor cell lines in culture with a wide variation in IC50 values (2.6-79 mumol/L, or 1-30 micrograms/mL). In only a few cases was the IC50 below 13.2 mumol/L (5 micrograms/mL), the presumed upper limit for the serum genistein concentration in those on a high soy diet. In future studies, greater emphasis should be placed on the effect of genistein on nontransformed, normal cell lines from the tissues where cancer can occur rather than established tumor cell lines. Similarly, the effect of genistein on the progression and/or promotion of cancer may be more clearly examined using nontransformed cell lines transfected with specific oncogenes thought to be activated during oncogenesis.

美国《营养学报》，1995年

金雀异黄素对体外和体内模型的影响

斯蒂芬 巴恩斯

阿拉巴马大学药理学系，伯明翰35294

在三分之二的关于含有染料木素的大豆物质在癌症动物模型中的作用的研究中，癌症的风险（发病率，潜伏期或肿瘤数）明显减少。此外，纯化的染料木黄酮延迟乳腺肿瘤的外观与细胞增多有关当用新生儿给药时，用7,12-二甲基苯并[a]蒽处理的大鼠乳腺组织中的分化抑制佛波酯 - 在皮肤癌模型中诱导H2O2产生，并在结肠癌模型中抑制异常隐窝形成。在体外模型中，染料木黄酮抑制培养物中人肿瘤细胞系的增殖，IC 50值差异很大（2.6-79μmol/ L或1-30微克/ mL）。在少数情况下，IC50低于13.2 mumol / L（5微克/ mL），血清染料木黄酮的推定上限在高脂饮食中的浓度。在未来的研究中，应更加重视染料木素对非转化，来自癌症可能发生的组织的正常细胞系而不是建立的肿瘤细胞系。类似地，染料木素的影响可以使用转染特异致癌基因的非转化细胞系更清楚地检查癌症的进展和/或促进认为在肿瘤发生过程中被激活。