Cancer Epidemiology, 2010, 34(2):200-6.

Genistein and daidzein act on a panel of genes implicated in cell cycle and angiogenesis by Polymerase Chain Reaction arrays in human prostate cancer cell lines

Martin Braud, Samir Satih, et al

CNRH, 58 rue Montalembert, 63009 Clermont-Ferrand, France; et al

BACKGROUND: The prostate cancer most frequently affects men. The ethnic origin and family antecedents of prostate cancer are established as risk factors. The genetic factors associated with environmental factors such as the nutrition also play a role in the development of the cancer. Epidemiological studies showed that the Asian populations exhibited an incidence of prostate cancer markedly subordinate by comparison with the Western populations. This would be explained partially by their important consumption of soy. Both main phytoestrogens of soy, the genistein and the daidzein, present anti-proliferative properties.

METHODS: For that purpose, we used different prostate cancer cell lines (LNCaP, DU 145, PC-3) and, by flow cytometry, we determined the concentration of phytoestrogens inducing a cell cycle arrest and the required time of incubation.

RESULTS: Then, the effects of 40microM genistein or 110microM daidzein for 48h were determined and studied on the expression of genes involved in the human cell cycle and angiogenesis and conducted by SYBR green quantitative PCR.

CONCLUSION: We demonstrated modulations of cyclin-dependent kinase-related pathway genes, DNA damage-signaling pathway and a down-regulation of EGF and IGF.

荷兰《癌症流行病学》，2010年4月

染料木素和大豆黄酮在人体前列腺癌细胞株中通过聚合酶链反应阵列对与细胞周期及血管生成牵连的基因组发挥作用

马丁 劳，萨米尔  赛斯等

法国克莱蒙费朗58 街蒙塔龙贝国家人权机构等

背景︰ 前列腺癌是一种最频繁影响男性的疾病。根据风险因素，确认了前列腺癌的种族起源和发展历程。遗传因素与环境因素，如营养等也在癌症发展中起到了作用。流行病学研究表明，亚洲人群前列腺癌的发病率明显小于西方人群。这一点可以以对大豆的重要消耗来部分地解释。大豆的两个主要植物雌激素-染料木素和大豆苷元，表现出抗增殖特性。

方法︰ 为此目的，我们使用了不同的前列腺癌细胞株 (LNCaP，DU 145， PC-3)，并且通过流式细胞仪，确定了植物雌激素诱导细胞周期阻滞的浓度和孵化所需的时间。

结果︰ 然后， 40 uM 染料木黄酮或 110 uM 大豆黄酮48 小时的作用被确定，并且通过SYBR绿色定量PCR对参与人体细胞周期和血管生成基因的表达进行研究。

结论︰ 我们证明了细胞周期蛋白依赖性激酶相关通路基因、 DNA 损伤信号通路和表皮生长因子和胰岛素样生长因子下调的变化。