Journal of Korean Medical Science, 2011, 26(10):893-9.

Antiproliferation and Redifferentiation in Thyroid Cancer Cell Line by Polyphenol Phytochemicals

Hee Joon Kang; et al

Department of Surgery, Hangang Sacred Heart Hospital, Hallym University College of Medicine, Seoul; et al

Thyroid carcinogenesis is accompanied by loss of thyroid-specific functions and refractory to radioiodine and thyroid stimulating hormone (TSH) suppression therapy. Redifferentiating agents have been shown to inhibit tumor growth and improve the response to conventional therapy. Polyphenol phytochemicals (PPs) in fruits and vegetables have been reported to inhibit cancer initiation, promotion, progression and induce redifferentiation in selected types. In this study we examined PPs induce redifferentiation in thyroid cancer cell lines. We investigated the effects of genistein, resveratrol, quercetin, kaempferol, and resorcinol on the F9 embryonal carcinoma cell differentiation model. The thyroid cancer cell lines, TPC-1, FTC-133, NPA, FRO, and ARO, displayed growth inhibition in response to genistein, resveratrol, quercetin. We further demonstrated that genistein decreased the dedifferention marker CD97 in NPA cells and resveratrol decreased CD97 in FTC-133, NPA, FRO cells and quercetin decreased CD97 in all cell lines. We observed increased expression of differentiation marker NIS in FTC-133 cells in response to genistein, and resveratrol but no change in NPA, FRO, ARO cells. Quercetin increased or induced NIS in FTC-133, NPA, FRO cells. These findings suggest that PPs may provide a useful therapeutic intervention in thyroid cancer redifferentiation therapy.

韩国《韩国医学科学杂志》，2011年4月

植物化学物质多酚对甲状腺癌细胞的抗增殖和再分化

康熙俊等

韩国首尔翰林大学医学院汉江圣心脏医院外科部

甲状腺癌伴随着甲状腺特异功能的丧失，并且放射性碘和促甲状腺激素 (TSH) 抑制疗法难以治愈。再分化试剂已被证明可以抑制肿瘤生长，提高其对常规治疗的反应。水果和蔬菜中含有的植物多酚 (PPs)被报道可以抑制癌症的萌生、 推广、 进展，并且诱导其向选定的类型分化。在本研究中，我们研究了PPs诱导甲状腺肿瘤细胞再分化。我们探究了金雀异黄素、 白藜芦醇、 槲皮素、 山奈酚，和间苯二酚对 F9 胚胎癌细胞分化模型的影响。甲状腺癌细胞，TPC 1、FTC-133、 NPA、 FRO和 ARO，染料木黄酮、 白藜芦醇、 槲皮素会对它们进行生长抑制。我们进一步表明，在NPA 细胞中染料木黄酮降低了去分化标记物 CD97 ，白藜芦醇在FTC-133、NPA、FRO细胞中降低了CD97，而槲皮素在所有细胞中降低了 CD97 。我们观察到在 FTC 133 细胞中金雀异黄素和白藜芦醇均会使分化标记物 NIS 的表达增加，但是在NPA、FRO、和ARO 细胞中没有变化。槲皮素增加或诱导FTC-133、NPA、FRO细胞中的NIS  。这些结果表明，PPs可能提供一种对甲状腺癌再分化治疗有用的治疗方法。