Romanian journal of morphology and embryology, 2011, 52(3 Suppl):1065-9.

A comparison regarding antiproliferative action between soy total extract and genistein

Corina Tiulea, et al

Department of Pharmacognosy, Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy, Timisoara, Romania.

The aim of this study is to make a comparison between the action of genistein and total soy extract regarding anticancer action on two different in vivo models: phytobiological test and animal model, and to see which of the two tested samples present a greater antiproliferative effect. Soybean seeds were grounded and a solvent formed of DMSO-ethanol-water in rapport 5-70-25 v/v/v was prepared. The extraction was made using an ultrasonic bath (Falc LCD Series) for 30 minutes, 59 kHz. The solvent was evaporated with a rotary evaporator at 50°C. Genistein was acquired from Extrasynthèse (France), hydroxypropyl-γ-cyclodextrin (HPGCD) from Cyclolab Hungary, 7,12-dimethylbenz[a]anthracene (DMBA), dimethylsulfoxide (DMSO), and 12-O-tetradecanoylphorbol-13-acetate (TPA) from Sigma Aldrich, Germany. Because of the poor water solubility, genistein was prepared in a complex with hydroxypropyl-γ-cyclodextrin in a molar ratio 1:2 by kneading method and total soy extract in a mass ratio 1:4 also by kneading method. Phytobiological test indicated an inhibition index over 50% in case of solutions of concentration between 8-33% in both samples, suggesting a possible antiproliferative action at a superior level. Study on C57BL/6J mice was made on which it was induced cancer with physical agents like DMBA, and it was promoted with TPA. Mice where divided in four groups: Group A - blank group, Group B - mice who received total soy extract, Group C - mice who received genistein, Group D - untreated mice. Results on animal model show that both soy total extract and genistein inhibited the initiation and promotion of chemically-induced skin tumorigenesis, but genistein had a greater success in recovering skin lesions type experimental malignant melanoma.

罗马尼亚《形态学和胚胎学罗马尼亚学报》，2011年

关于大豆总提取物与染料木黄酮抗增殖作用的比较

科瑞娜 蒂乌莱亚等

罗马尼亚维克多医药大学药学院药学管理系等

本研究的目的是比较染料木黄酮和总大豆提取物对两种不同的体内模型的抗癌活性︰植物醇生物试验和动物模型，来确认这两个测试样本目前抗增殖作用更大的一个。研磨大豆种子并制备一种浓度为5-70-25 v/v/v的二甲基亚砜-乙醇-水溶剂。以59kHz的功率超声30 分钟（Falc LCD系列）。用旋转蒸发仪在 50℃蒸发溶剂。从 Extrasynthèse （法国）购买金雀异黄素，从匈牙利 Cyclolab购买羟丙基-γ-环糊精 (HPGCD)，7,12-二甲基苯并 [a] 蒽 (DMBA)，二甲基亚砜 (DMSO) 并且从德国西格玛奥德里奇购买12-O-十四烷酰佛波醇-13-醋酸酯 (TPA)。由于水溶性差，以1:2的摩尔比将金雀异黄素和羟丙基-γ-环糊精通过揉捻法混合在一起，而总大豆提取物按照质量比 1:4 也混合在一起。植物醇生物试验表明当两种样品的浓度位于8-33%之间时抑制指数超过 50%，展现了最高水平的抗增殖活性。对 C57BL/6J 小鼠进行研究，利用物理试剂 DMBA诱发的癌症并且用TPA加快癌症进程。小鼠被分为四组︰ A 组-空白组，B 组-接受总大豆提取物的小鼠，C 组-接受金雀异黄素的小鼠，D 组-未经处理的小鼠。动物模型结果表明大豆总提取物和染料木黄酮都能抑制化学诱导皮肤肿瘤的发生和发展，但是金雀异黄素在恢复实验恶性黑色素瘤造成的皮肤损伤方面具有更好的效果。