Lasers in Surgery and Medicine, 2012, 44(10):840–849.

Combination with Genistein Enhances the Efficacy of Photodynamic Therapy Against Human Anaplastic Thyroid Cancer Cells

Jin Chul Ahn, Raktim Biswas, Phil Sang Chung

Medical Laser Research Center, College of Medicine, Dankook University, 29-1, Anseo-dong, Cheonan, Chungnam 330-714, Korea

BACKGROUND AND OBJECTIVE:

Photodynamic therapy (PDT) has become one of the emerging options in management of cancer and other diseases. The major goal of PDT is to kill cancer cell without causing any adverse effect to the normal cells. PDT in combination of different therapeutic agents is being evaluated to improve the efficacy of treatment. Genistein, a soy ingredient, has widely been studied against different types of cancer. In the present study, combination of these two therapeutic methods has been studied to evaluate the enhanced effectiveness and find out the mechanism of action.

MATERIALS AND METHODS:

Combination of PDT and genistein has been studied against human thyroid cancer cells SNU 80. Cells were treated with genistein and different concentration of photofrin in PDT singly and conjointly. Viability of SNU 80 cells was analyzed using MTT assay. The cells were stained with Hoechst 33342 and propidium iodide (PI) for morphological observations. Changes in mitochondrial membrane potential and generation of reactive oxygen species (ROS) were also studied by confocal microscopy. Western blot analysis were also performed to find out the expressions of different pro- and anti-apoptotic proteins.

RESULTS:

From the result, the combination of genistein and photofrin mediated PDT enhanced the apoptotic effect against SNU 80 cells. Proliferation of the cells was inhibited and the mitochondrial membrane depolarisation was observed. ROS level were also increased in combination treatment. The expressions of Caspase 3, Caspase 9, cytochrome c, Caspase 8, Caspae 12 and other apoptosis related proteins were also modified.

CONCLUSION:

Thus PDT can induce apoptosis in thyroid cancer cells singly or in combination with genistein. But in combination treatment, the efficacy of inducing apoptosis in SNU 80 cells is much higher than that of individual treatment with genistein or PDT.

美国《激光手术和医学》，2012年12月

金雀异黄素增强了光动力疗法对人体甲状腺未分化癌细胞的疗效

安金澈， 瑞克提姆 比斯瓦斯，菲尔 桑 钟

韩国檀国大学医学院医疗激光技术研究中心

背景与目的︰

光动力疗法 (PDT) 已经成为治疗癌症和其他疾病的新兴选项之一。PDT的主要目标是杀死癌细胞并且不会对正常细胞造成任何不利影响。测试PDT与不同的治疗药剂的联合方法，来提高治疗的疗效。金雀异黄素，一种 大豆成分，对不同类型癌症的作用得到广泛研究。在本研究中，研究了联合这两种治疗方法增强的成效，发现其作用机理。

材料和方法︰

研究光动力疗法和金雀异黄素的组合对人体甲状腺癌细胞 SNU 80的作用。用金雀异黄素和PDT不同浓度的光敏剂单独并连续地治疗细胞。用 MTT 法分析SNU 80 细胞的活性。细胞上沾满 Hoechst 33342 和碘化丙啶 (PI) 用于形态学观察。用共焦显微镜研究了线粒体膜电位和生成的活性氧 (ROS) 的变化。通过免疫印迹分析，找出不同的预和抗凋亡蛋白的表达。

结果︰

结果表明，金雀异黄素和光敏剂的组合介导的PDT提高了对SNU 80 细胞的凋亡作用。细胞增殖被抑制，并观察线粒体膜的去极化。在组合治疗法中活性氧水平也有所提高。半胱氨酸蛋白酶 3、 半胱氨酸蛋白酶 9、 细胞色素 c、 半胱氨酸蛋白酶 8、半胱氨酸蛋白酶12 和其他细胞凋亡相关蛋白的表达也进被修改。

结论︰

因此PDT可以诱导甲状腺肿瘤细胞凋亡单独或者与金雀异黄素结合。但是在联合治疗中，诱导 SNU 80 细胞凋亡的疗效远高于用金雀异黄素或 PDT 的单独治疗。