Iranian Journal of Immunology, 2012, 9(2):119-27.

Genistein Induces a Protective Immunomodulatory Effect in a Mouse Model of Cervical Cancer

Amir Ghaemi, Hoorieh Soleimanjahi, Soodeh Razeghi, et al

Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran; et al

BACKGROUND:

Genistein (GEN), a naturally occurring flavonoid present in soy bean, has attracted scientific interest for its possible benefits in cancer.

OBJECTIVE:

The potential immunomodulatory effects of genistein on the immune system and against TC-1 tumor cell line were evaluated in adult female C57BL/6 mice.

METHODS:

Mice were treated with GEN 10 days before to 10 days after the tumor induction. Thirty days after the last GEN treatment, lymphocyte proliferation, Lactase Dehydrogenase (LDH) cytolytic activity and cytokine secretion were analyzed in GEN and control groups.

RESULTS:

The results showed that ingestion of genistein significantly increased lymphocyte proliferation and LDH release. Furthermore, the treatment with genistein also caused a significant increment in interferon gamma (IFN-γ). In addition, the treatment achieved significant therapeutic effect in tumor models compared to the control group. These results indicated that the effect of GEN on tumor growth may be attributed to its effect on lymphocyte proliferation, cytolytic activity and IFN-γ production.

CONCLUSION:

These results demonstrate that GEN exerts an immunomodulatory effect in a mouse model of Human Papillomavirus (HPV) associated-cervical cancer.

伊朗《伊朗免疫学杂志》，2012年6月

在患宫颈癌的小鼠模型中金雀异黄素诱发了保护免疫调节作用

阿米尔 格哈伊米，哈瑞埃士 捜雷曼雅诗，苏丹 拉齐格等

伊朗戈勒斯坦医科大学医药学院等

背景︰

金雀异黄素 （GEN），在大豆中存在的天然黄酮类化合物，由于其对癌症可能的益处吸引了科学界的兴趣。

目的︰

在成年雌性 C57BL/6 小鼠中评价金雀异黄素潜在的免疫调节效应对免疫系统和 TC-1 肿瘤细胞的影响。

方法︰

在诱导出肿瘤10 天后对小鼠进行 10 天的金雀异黄素治疗。在GEN治疗三十天后，分析GEN组和对照组的淋巴细胞增殖、 乳糖酶脱氢酶 (LDH) 溶细胞活性和细胞因子分泌等。

结果︰

结果表明金雀异黄素摄入显著提高了淋巴细胞的增殖和乳酸脱氢酶的释放。此外，用金雀异黄素治疗同样会使 γ 干扰素 （IFN-γ）大幅度增加。而且，对照组相比，在肿瘤模型中治疗取得了显著疗效。这些结果表明，GEN对肿瘤生长的影响可能归因于其对淋巴细胞增殖、 溶细胞活性和 IFN-γ 生成的影响。

结论︰

这些结果表明在小鼠模型中GEN对人体乳突病毒 (HPV) 相关的宫颈癌具有免疫调节作用。