针对病种：癌症
发表时间：2017年2月
发表国家：中国
登载刊物：生物化学进展
研究单位：美国田纳西州亨德森西田纳西州癌症研究所; 等
研究人员：萨恩威特 JT，赛斯 HR，英格兰度 SR，等
主要结论：开发水溶性混合物的抗癌化合物在NNS中具有高生物利用度（> 90％）并且不会在胃中降解以预防和治愈癌症.
Advances in Biological Chemistry, 2017, 07(1):27-41.
Anticancer Effects of Curcumin, Artemisinin, Genistein, and Resveratrol, and Vitamin C: Free Versus Liposomal Forms
Thornthwaite J T, Shah H R, England S R, et al
Cancer Research Institute of West Tennessee, Henderson, TN, USA; et al
Cancer prevention supplements, which also provide effective treatment with minimal side effects, are urgently needed. An accurate, fast assay system is described that reveals the ability of chemically defined products, such as curcumin, genistein, resveratrol, artemisinin, and vitamin C, to kill K562 Erythroleukemic cells in vitro. In addition, curcumin and vitamin C were encapsulated into fatty acid micelles named Nutra Nano Spheres TM (NNS) using all natural products. A unique viability stain, which allows the rapid staining of dead cells by membrane penetration using Propidium Iodide, was used to measure the cell viability by flow cytometry. Cell death by alteration of the cell membranes could be seen within 30 s of exposure to curcumin. The other free components required 0.5 - 70 h to see maximum killing, suggesting a more metabolic and/or apoptotic route of cancer cell destruction. Vitamin C up to 1 × 104 µmol/well did not affect K562 cell viability. The vitamin C-NNS (3.2 nm diameter-60 mg/50 µL) showed an LD50 = 133 µmol/well ± 11 SD (n = 4), which was over 75 times more potent than the free vitamin C. The curcumin-NNS (7.4 nm diameter-25 mg/50 µL) resulted in an LD50 = 41.3 µmol/well ± 5.6 SD (n = 8) and represented a 264 fold increase in activity to destroy the cancer cells. The clinical goal is to develop water-soluble mixtures of anti-cancer compounds in the NNS with their high bioavailability (>90%) and without degradation in the stomach for preventing and curing cancer.
中国《生物化学进展》，2017年2月
姜黄素，青蒿素，染料木素，白藜芦醇和维生素C：自由与脂质体形式的抗癌作用
萨恩威特 JT，赛斯 HR，英格兰度 SR，等
美国田纳西州亨德森西田纳西州癌症研究所; 等
癌症预防补品，也提供有效的治疗最小的副作用是迫切需要的。准确，快速的分析系统是描述了揭示化学定义的产品如姜黄素，染料木素，白藜芦醇，青蒿素和维生素C在体外杀死K562红细胞白血病细胞的能力。此外，姜黄素和维生素C被包裹在名为NutraNanoSpheresTM（NNS）的脂肪酸胶束中，使用全部天然产品。独特的活力染色，可以快速染色使用碘化丙啶通过膜渗透的死细胞被使用通过流式细胞术测量细胞活力。细胞因细胞死亡而改变在暴露于姜黄素的30秒内可以看到膜。另一个免费组件需要0.5 - 70小时才能看到最大的杀戮，提示更多癌细胞破坏的代谢和/或凋亡途径。维生素C达1×104μmol/孔不影响K562细胞活力。维生素C-NNS（3.2nm直径-60mg /50μL）显示LD 50 =133μmol/孔±11SD（n = 4），其比游离维生素C的效力高出75倍以上。姜黄素-NNS（7.4nm直径-25mg /50μL）导致LD 50 = 41.3μmol/孔±5.6SD（n = 8），活性增加264倍破坏癌细胞。临床目标是开发水溶性混合物的抗癌化合物在NNS中具有高生物利用度（> 90％）并且不会在胃中降解以预防和治愈癌症。
