Global Journal of Medicine Researches and Studies，2017
Effect of Genistein on DNMT1 Gene Expression and Cell Proliferation of Hepatocellular Carcinoma HepG2 Cell Line
Masumeh Sanaei, et al
Research Center for Non-communicable Diseases, Jahrom University of Medical sciences, Jahrom, Iran; et al
Hepatocellular (HCC) carcinoma is one of the most common types of cancer and the major form of primary liver cancer. Genetic and epigenetic changes play a significant role in tumourigenesis. Deregulation of epigenetic patterns leads to cancer in deferent tissues. Epigenetic alterations are reversible by epi-drugs. DNA methyltransferases (DNMTs) catalyze DNA methylation and directly responsible for hypermethylation of tumorsuppressor genes. Genistein (GE) has been shown to decreases methylation of DNA promoter of tumor suppressor genes by reduction of DNMT1 activity by which suppress the growth of various cancers. The aim of the present study was to analyze the effect of GE on cell growth and DNMT1 gene expression in the hepatocellular carcinoma HepG2 cell line. Materials and Methods: MTT assay and Real-Time Quantitative RT-PCR were used to evaluate proliferative effect and DNMT1 gene expression. Results: GE inhibited the growth of HepG2 cells and decreased DNMT1 gene expression significantly with a time- and dose-dependent manner. Discussion: Our finding clearly indicated that GE has a significant inhibitory effect and decreases DNMT1 gene expression significantly. Conclusion: GE can significantly inhibit the growth of HepG2 cells and plays a significant role in DNMT1 gene expression.
《全球医学研究与研究杂志》，2017年
金雀异黄素对DNMT1基因表达和肝细胞癌HepG2细胞增殖的影响
马苏么 森尼 ，等
伊朗Jahrom医学科学院非传染性疾病研究中心，等
肝细胞癌（HCC）是最常见的类型之一癌症和原发性肝癌的主要形式。遗传和表观遗传学变化在肿瘤发生中起重要作用。放松管制表观遗传模式导致不同组织中的癌症。表观遗传改变被外用药物所逆转。 DNA甲基转移酶（DNMTs）催化DNA甲基化，直接负责肿瘤抑制基因的超甲基化。染料木黄酮（GE）已显示降低甲基化的DNMT1减少肿瘤抑制基因的DNA启动子抑制各种癌症生长的活动。的目的目前的研究是分析GE对细胞生长和DNMT1的影响基因表达在肝细胞癌HepG2细胞株中。物料方法：采用MTT法和实时定量RT-PCR评估增殖效应和DNMT1基因表达。结果：GE抑制HepG2细胞生长，降低DNMT1基因以时间和剂量依赖的方式显着表达。讨论：我们的发现清楚地表明，GE具有重要意义DNMT1基因表达显着降低。结论：GE可显着抑制HepG2细胞的生长在DNMT1基因表达中起重要作用。
