针对病种：宫颈癌
发表时间：2018.1.1
发表国家：
登载刊物：Journal of Cancer 2018, Vol. 9《癌症杂志2018》，第9卷
研究单位：食品科学技术国家重点实验室，南昌大学
研究人员：陈海红，陈淑萍，郑秋玲，聂绍平，李文娟，胡晓娟，谢明勇
主要结论：染料木素(Gen)降低众多 癌症包括乳腺癌，宫颈癌和前列腺癌的发病率.
Phytoestrogens are polyphenol compounds which have similar structure to 17β-estradiol (E2), a
kind of main estrogen in women. Thus, phytoestrogens may affect the reproductive and endocrine
systems, leading to the development of estrogen-related cancers. The effect of genistein (Gen), one
of the most studied phytoestrogens, on human cervical cancer cells (HeLa) was investigated in this
study. It was found that Gen at concentrations of 0.001, 0.01, 0.1 and 1 μmol·L-1 promoted the
proliferation of HeLa cells in a dose-dependent manner. Gen increased the portion of HeLa cells in
S phase and decreased the portion of the cells in G1 phase. Besides, apoptosis rate of the cells was
significantly lower when treated with Gen compared with the control group. It was also found that
the expression of ERα, Akt or nuclear NF-κB p65 protein was activated by Gen. The correlation
between these three proteins may be as following: ERα was the upstream, followed by Akt, and then
nuclear NF-κB p65 protein. In addition, the downstream genes of activated nuclear NF-κB p65 were
found to be associated with cell cycle and apoptosis of cancer cells. Our results suggested that Gen
may stimulate cell proliferation partially through the estrogen receptor-mediated PI3K/Akt-NF-κB
pathway and the further activation of the downstream genes of nuclear NF-κB p65.
染料木素(Gen)对人类宫颈癌细胞(HeLa)的作用进行了研究。发现Gen增加了HeLa细胞的比例S期，G1期细胞比例降低。细胞凋亡率为与对照组相比，Gen组明显降低。我们的结果表明，Gen可能会刺激细胞增殖部分通过雌激素受体介导PI3K / Akt-NF-κB下游基因的进一步激活核NF-κB p65加速癌细胞的凋亡
